[Microbiology] Re: Culture media and temperature

Bob bbx107 at excite.XXXX.com
Fri Nov 25 21:35:05 EST 2005


On 24 Nov 2005 22:47:30 -0800, ahomosapien at gmail.com wrote:

>Hi,
>Can anyone tell me whether fresh LB is better for bacterial growth than
>LB made the previous day, refrigerated overnight and used after warming
>to room temp? there should not be any problem, isnt it? IS any
>detrimental effect known at all?

I'd be surprised if LB was much different after one day storage.
However, I would note that room temperature storage is probably more
common than refrigerator.

Note that if there is any difference, it might be for the better or
for worse. There is no logical reason why, if they differ, it is
predictable which would be better. If you are growing some finicky bug
and think media freshness might be an issue, try things -- with an
open mind.

bob




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