[Microbiology] Re: Culture media and temperature

Tom Knight tk at csail.mit.edu
Fri Nov 25 21:42:39 EST 2005


ahomosapien at gmail.com writes:

> Can anyone tell me whether fresh LB is better for bacterial growth than
> LB made the previous day, refrigerated overnight and used after warming
> to room temp? there should not be any problem, isnt it? IS any
> detrimental effect known at all?

LB composition and quality are poorly controlled.  There is a great
deal of variation between batches of LB, which may explain differences
you have observed.  We keep LB in the refrigerator for 2-3 months with
little apparent effect.



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