[Microbiology] M56 minimal medium.

Monalisa Sunkara msunkara at uncc.edu
Thu Sep 29 15:20:14 EST 2005


hi,
I am trying to grow E.coli in M56 salt medium supplemented with glucose 
by picking a colony from 2-3 days old E.coli SM agar plate and 
incubating in 10 ml of  M56 o/n at 37 degress and 187 rpm as mentioned 
in literature.But i am not getting OD (550nm)=2 after o/n incubation as 
is mentioned in the literature.Rather i am getting OD of only 0.2.Any 
suggestions?



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