[Microbiology] Re: Help needed

Bob bbx107 at excite.XXXX.com
Sun Apr 16 21:21:53 EST 2006


On Sat, 15 Apr 2006 22:32:49 -0700 (PDT), kondepudi kiran
<kanthi_kiranin at yahoo.com> wrote:

>Dear Sir
>   
>  My question is
>   
>  1.  how to do electrphoresis and zymogram (activity satining of enzymes on electrophoretic gels) of extracellular enzymes from obligate halophiles since high NaCl possess problems in electrophoretic mobility.
>   
>  2. How to purify extracellular hydrolytic enzymes like amylase etc which needs compulsory NaCl for the activity
>   


Why do you think there is a problem?? Unless the enzyme is
irreversibly denatured by low salt, it would not be a problem. Do you
know that it is?

Why is the salt a problem during purification??

bob




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