[Microbiology] Re: methylene blue milk

JEDilworth bactitech at nospamhortonsbay.com
Mon Aug 14 10:09:03 EST 2006


I worked in a very small micro lab in the 70's and they were using a big 
pickle jar and a candle for their chocolate and Thayer Martin plates. It 
worked quite well. It was messy though, and somewhat tricky to position the 
candle and light it so you wouldn't burn yourself.

I doubt whether it would be allowed in our more safety conscious 
laboratories now.

I believe you meant 5% CO2 conditions, not anaerobic conditions. You need 
gas generators for the latter.

Judy Dilworth, M.T. (ASCP)

"N10" <limbic_lesion at hotmail.com> wrote in message 
news:NvidnbOphZo5rX3ZRVnyig at bt.com...

> I remeber once just a few years back when we ran out of CO2 for the 
> genereation of  anaerobic conditions  I reverted to candling ! and it 
> worked very staisfactorily much to the amazement of the younger 
> microbiologists who witnessed it.




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