[Microbiology] Re: enumeration of non-cultureable bacteria

myrehfuss at ucdavis.edu myrehfuss at ucdavis.edu
Thu Aug 17 16:45:45 EST 2006


biovirus04 at gmail.com wrote:
> That's partially what I didn't get.  Why would E. coli be
> non-culturable?
>
> --Alex

Stress and low pH can induce a VBNC state in E. coli.  Perhaps NaCl and
osmotic stress (or some other stress) in seawater can do likewise?
VBNC E coli are apparently found in aquatic environments.

Another method of enumeration that requires no culture would be FISH
using E coli specific probes. 

Marc



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