[Microbiology] Boron Tolerance in Bacteria
(by iftikharnarc At hotmail.com)
Tue Dec 5 10:08:05 EST 2006
Dear Professionals in microbiology,
I hope, you will be fine and healthy.
I am working on Boron Toxicity tolerance in Bacteria and I isolated several novel B-tolerant species. Now I am studying Boron Tolerance Mechanism, through B-uptake studies.
I want your kind help to understand an issue in my data. Kindly spare some moments from your busy schedule to see my data as here in the attached file "Data-uptake studies". Here I explain the problem that I could not understand although I repeated this experiment several times.
While doing B-uptake studies in bacteria (data is attached with figures, short introduction, and Materials and Methods and also with a short results and discussion), one species Bacillus boroniphilus (characterized and published just recently, please see characteristics of this species from the attached file "MS-boroniphilus") that requires B for its growth (unique character of this species in bacteriology) and at the same time, can tolerate very high concentration of B, showed more membrane (pallet)-bound B when exposed to low level of B supply than when exposed to high level (please see figure 1b, the last bars, page 7 in the attached data file). I could not understand the reasoning for this aspect. I repeated this experiment several times but always found same results i.e. more pallet-bound B when exposed to low B supply compared to when exposed to high B supply. In all other strains, the trend is opposite and understandable.
I would be thankful and obliged of your kindness and cooperation to extend my view for this data.
My best regards
Biotechnology Research Centre,
The University of Tokyo
More information about the Microbio