[Microbiology] Zymogram for amylase

ashabil ashabil at cu.edu.tr
Fri Sep 1 09:38:44 EST 2006


Hi,
I m having a problem with amylase zymogram analysis. Although i had 
positive result on plate (starch in agar, dropped 30 microlitre partially
purified enzyme), i could not get any positive result on gel( both sds and
native gel). 

Can anyone please advise me a protocol, at least for renaturation of enzyme.
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