[Microbiology] Re: Enrichment of biofilms producing anaerobes

GS not at home.com
Tue Sep 26 14:54:22 EST 2006


"Bob" <bbx107.XYZ at excite.XYZ.com> wrote in message 
news:qn6hh2ljdeqk9hcom4ijnt5qrr9jifl10i at 4ax.com...
> On Mon, 25 Sep 2006 14:34:42 -0400, "Matt McMahon"
> <matt.mcmahon at chee.queensu.ca> wrote:
>
>>
>>
>>Hi I was wondering if anyone had any references/suggestions for
>>selective enrichment of biofilm producing anaerobic bacteria.  I am
>>familiar with standard selective enrichment techniques in suspended
>>culture and that most bacteria will form biofilms EVENTUALLY if given
>>the proper conditions.  My goal is to enrich for bacteria that are more
>>predisposed to form biofilms compared to  a pure reference culture that
>>I am working with.  My initial thoughts are to work with serum bottles
>>filled with a pelletized material to provide additional surface area for
>>biofilm formation and before transfer to agitate the bottles so attached
>>cells are loosened and can be transferred via syringe.
>>
>>
>>So in short, classical enrichment techniques favour suspended growth
>>bacteria with the fastest kinetics.  Is there any easy way to enrich for
>>bacteria with strong biofilm tendencies.
>>
>
> So collect the bacteria from the surfaces, not from the suspension.
>
> There are lots of questions about details. To a large extent, those
> get answered as you formulate the question.
>
> bob
>

How about a fluidised bed configuration? eg a column packed with sterilised 
particles of a suitable size with the medium recirculated through the 
column?

GS 




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