From dietz from tu-harburg.de Mon Dec 3 06:53:24 2007 From: dietz from tu-harburg.de (David Dietz) Date: Mon Dec 3 11:26:01 2007 Subject: [Microbiology] Synthetic medium for methanosarcina Message-ID: <4753EE34.5050007@tu-harburg.de> Hello, does anyone know, if there is a methanosarcina-strain that grows on a synthetic medium with a sensible growth rate - without addition of vitamins or complex medium components. I want to ween my M. mazei Goe1 to acetate from methanol, but up to now I was not successful. Did anyone of you observe similar problems and can you tell me, how you solved the problem? That would be a great help! David From dietz from tu-harburg.de Mon Dec 3 08:00:16 2007 From: dietz from tu-harburg.de (David Dietz) Date: Mon Dec 3 11:26:05 2007 Subject: [Microbiology] methanosarcina cultivation Message-ID: <4753FDE0.7010301@tu-harburg.de> Hello, does anyone know, if there is a methanosarcina-strain that grows on a synthetic medium with a sensible growth rate - without addition of vitamins or complex medium components. I want to ween my M. mazei Goe1 to acetate from methanol, but up to now I was not successful. Did anyone of you observe similar problems and can you tell me, how you solved the problem? That would be a great help! David From txl8326 from louisiana.edu Wed Dec 5 15:19:44 2007 From: txl8326 from louisiana.edu (Luzan Tatiana) Date: Thu Dec 6 19:12:39 2007 Subject: [Microbiology] tetrathionate synthesis Message-ID: <20071205201153.M10455@louisiana.edu> Hello, I am trying to synthesize the potassium tetrathionate according to the recipie in P.A. Trudinger, 1961. Thiosulfate Oxidation and Cytochromes in Thiobacillus X. 2. Thiosulfate oxidizing enzyme. Biochem. J. 78, 680. The recipie says literally: 50 g of sodium thiosulfate (Na2S2O3,5H2O) was dissolved in 100ml of water and oxidized to completion with iodine. An excess of potassium acetate was added and K2S4O6 precipitated by the addition of 4 volumes of absolute ethanol. The material was rtecrustalized from 0.5N HCl. When I tried to do that, I could not oxidize the thiosulfate solution. First of all, the iodine we have is not readily soluble in water. Could you please help with the reagent names and preparation? I also appreciate if anyone will send me a different recipie for the tetrathionate synthesis with explanations. Thanks a lot Tanya (txl8326@louisiana.edu). -- Tanya Luzan Research Assistant Department of Biology University of Louisiana at Lafayette Phone (Office): 482-5056 From guruviswam from yahoo.com Thu Dec 13 06:11:53 2007 From: guruviswam from yahoo.com (redsa ewwdd) Date: Thu Dec 13 13:44:50 2007 Subject: [Microbiology] riboflavin production Message-ID: <414654.53581.qm@web94914.mail.in2.yahoo.com> Dear Sir Greetings. I understand from the scientific journals that you are doing lot of research projects related to vitamin production using microbes.. I would like to inform that I like to carry out a pilot project on production of riboflavin in laboratory using probiotics bacteria "for which I need your help to collect the related information/ procedure for determination / assay of vitamin B2 produced through bacterial fermentation (.i.e. I want to study the production of riboflavin in laboratory by using bacteria such as Lactobacillus. Leuconostoc. Bifidobacterium . I want to quantitatively estimate the vitamin produced in the cultures .. Please indicate the procedure or method available for determination like fermenatation medium, identificaton of vitamin in medium, recovery quantitative assay etc,,.Please send me the detailed procedure through my email id. With kind regards yours sincerely. Dr.K.Viswanathan, M.V.Sc., Ph.D. Professor, Dept. Of Animal Nutrition, Madras veterinary college, Chennai Tamilnadu , India Did you know? You can CHAT without downloading messenger. Go to http://in.messenger.yahoo.com/webmessengerpromo.php/ From tcaetano from ua.pt Tue Dec 18 19:43:02 2007 From: tcaetano from ua.pt (=?ISO-8859-1?Q?T=E2nia_Caetano?=) Date: Tue Dec 18 22:52:28 2007 Subject: [Microbiology] "cryptic plasmid?!" Message-ID: <15490469-9497-496A-AA2F-8FA828605972@ua.pt> Hello Anyone knows if it is possible a bacterial cell with a plasmid survive without it? In other words, there is any case of a plasmid essential to the bacterial growth (environmental sampling)? And if it is the case we can apply the term cryptic ? Because I thought cryptic was just a plasmid with unknown function. thanks biotc