[Microbiology] Filamentous bacteria identification

Barbara Beavers via microbio%40net.bio.net (by barbea1 At comcast.net)
Sun Feb 4 20:19:43 EST 2007


Larry,
I found a question you had answered regarding filamentous bacteria on  
bio.net. I would really appreciate your help if you have a minute to  
answer a  question. I have isolated an organism  from a polymer  
emulsion (a raw material) that appears to be a filamentous gram neg  
rod. This organism will only grow on low pH media and it grows very  
slowly.
My question:
When I gram stain from a subculture after 24 hours  growth the cells  
are all free swimming with a few chains of cells. After 72 hours the  
filaments are starting to form. The appearance of sheaths are  
apparent at 72-96 hours. After 96 hours filaments are predominant,  
most are sheathed.
Is it  a proper technique when identifying  filamentous bacteria to  
observe older cultures? Am I correct in identifying this as a  
filamentous bacteria?
Thank you so much!
Barbara



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