[Microbiology] Re: identification Streptococcus bovis

JEDilworth via microbio%40net.bio.net (by bactitech from nospamhortonsbay.com)
Tue Feb 20 00:19:00 EST 2007


I assume you're plating this on a selective medium that would get rid of 
gram negatives, i.e. CNA blood agar (colistin/Nalidixic Acid added to 
Columbia blood agar base - it's a common medium in clinical labs). I would 
also plate on 5% TSA sheep blood agar but you'd probably pick up gram 
negatives with this also.

Pick gamma or dark alpha/gamma colonies to a new blood plate (pick only ONE 
colony to keep it pure). Label your isolates somehow so you know which one 
is which. They should be catalase negative gram positive cocci. The classic 
ID method for differentiating enterococci and Strep bovis is using bile 
esculin agar and 6.5% salt broth. Once you have a plate full of overnight 
growth you can inoculate a BE slant and a tube of salt broth for each colony 
you pick. Incubate overnight. S. bovis should be BE+/6.5% NaCl -. 
Enterococci are positive for both.

If you are using PYR discs, the protocol for S. bovis PYR negative--->BE 
slant overnight. If PYR neg/BE+ then you probably have a S. bovis and can 
continue on with further testing. I personally like the old fashioned 
BE/NaCl broth method. You can cheaply screen a lot of colonies this way and 
it is clear cut to read.

Once you screen via the BE/NaCl you can go on to further ID by API or GPI 
Vitek Card if you'd like.

Judy Dilworth, M.T. (ASCP)
Microbiology

"tere" <teremxr from hotmail.com> wrote in message 
news:1171883426.012915.119330 from l53g2000cwa.googlegroups.com...
> Hi,
>
> I'm trying to identifiy Streptococcus bovis from colon tissues but I
> can't find a good protocol before use API strep test ( Biomerieux) to
> finally isolated it.
> please, do you know any protocol to identify it in the laboratory by
> classic methods?
>
> thank you
> 




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