[Microbiology] Minimal media XLI-blue

Laurie Lentz-Marino via microbio%40net.bio.net (by lelentzm from mtholyoke.edu)
Thu Mar 8 12:35:08 EST 2007


I have had the same problem. WHat are the 'other supplements' you  
refer to? Glutamine, somthing else? THanks, LAurie  
lelentzm from mtholyoke.edu
 >I have been experiencing some problems with growing E. coli in M9
 >minimum media.  I tried to grow JM109 and XL1-Blue cells in M9 +
 >glucose.  Neither grew.  I did not find any difficiency in terms of
 >their genotype.  How can I get around with this problem?
 >I appreciate your kindly help.

 >Haishan

Did you include thiamine?  Both those strains require it.  I make and
filter-sterilize a 3.4 mg/ml stock solution (of thiamine
hydrochloride) which is 200x.  I add it, along with glucose and other
supplements, after autoclaving and cooling the media (and for plates,
just before pouring).  I store the stock solution frozen.

Hope this helps!
Bruce Amsden



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