From skinnerd from gmail.com Sun Nov 11 08:48:47 2007 From: skinnerd from gmail.com (skinnerd@gmail.com) Date: Sun Nov 11 12:56:18 2007 Subject: [Microbiology] Complete newbie question - Texas Red Message-ID: <1194788927.632243.287050@o38g2000hse.googlegroups.com> Hi, I should say from the outset that I know practically nothing about microbiology - I was just after a substance that fluoresces at visible wavelengths, and is excited by light at round 589nm. A quick google suggests that there's some stuff called Texas Red which does this, and that it's used by you sorts of guys. The thing is, I'm wanting to cover objects in it, rather than peering down a microscope. So... errr... is this expensive stuff to buy in quantity? Is it stable over long periods? Is the effect reasonably bright - i.e. if I coated something in it and shone a sodium light at it, would it shine red? Or am I barking up completely the wrong tree here? Feel free to ignore the question if it's as boneheaded as I suspect! From alirezabedeltavana from yahoo.com Mon Nov 12 03:05:59 2007 From: alirezabedeltavana from yahoo.com (ALIREZA BEDELTAVANA) Date: Mon Nov 12 16:29:35 2007 Subject: Fwd: FW: [Microbiology] A set of tests for Pseudomonas florescence Message-ID: <360223.17931.qm@web45106.mail.sp1.yahoo.com> Dear Sir I am working on the contamination of Milk with Gram-negative psychrotrophic spoilage bacteria and I should diagnose and differentiate different species of this bacteria. I can not use Commercial kit (like API 20 NE )and I have to make a smaller set of biochemical tests by myself. Because some of these biochemical tests (Like in API 20 NE) may not be necessary to diagnose this microorganism, specially pseudomonas florescence I prefer the set be smaller and more convenient. Any help would be GREATLY appreciated! Best Regards Alireza Bedeltavana alirezabedeltavana@yahoo.com Note: forwarded message attached. --------------------------------- Yahoo! Answers - Get better answers from someone who knows. Tryit now. From washingtonracheal63 from gmail.com Tue Nov 13 02:07:27 2007 From: washingtonracheal63 from gmail.com (Racheal Washington) Date: Tue Nov 13 12:49:53 2007 Subject: [Microbiology] student wanting to become a Microbiologist Message-ID: http://www.microbeworld.org/scientists/how.aspx Here is a website for you, good reading. How to Become a Microbiologist Career Profiles Anyone with an interest in science and the desire to explore the mysteries of life can become a microbiologist. You can, too. Here's your chance to see what some microbiologists have to say about their work, why they do it and how they came to be scientists. Reading their profiles, you can get a sense of what it's like to be a microbiologist and maybe pick up some tips on how you can pursue a science career. Ra?l Cano, Ph.D., the scientist who revived 30-million-year-old bacteria from the gut of a bee entombed in amber Cathy Squires, Ph.D., a microbiologist at Tufts University whose family farm offered her first experiences with microbes. Karen Nelson, Ph.D., leader of the team that unraveled the genetic code of a heat-loving, marine-dwelling bacterium called Thermotoga maritima at The Institute for Genomic Research Cliff Houston, Ph.D., bacteriologist and professor at the University of Texas Medical Branch This site is ICRA labeled. Copyright (c) 2006 American Society for Microbiology Visit our other ASM Sites: ASM.org for Scientists MicrobeLibrary.org for Educators Microbeworld thanks its sponsors: From rohitash13 from gmail.com Tue Nov 13 11:50:56 2007 From: rohitash13 from gmail.com (rohitash) Date: Tue Nov 13 17:36:33 2007 Subject: [Microbiology] Re: A set of tests for Pseudomonas florescence In-Reply-To: References: Message-ID: <1194972656.307392.158660@d55g2000hsg.googlegroups.com> On Oct 14, 7:07 am, "JEDilworth" wrote: > http://findarticles.com/p/articles/mi_m3301/is_3_107/ai_n16111788 > > I found this in about two seconds on google. > > Why Pseudomonas? The organism Ps. aeruginosa is the only one that > produces green pigment, pyocyanin, and is oxidase positive. Other > pseudomonads are more difficult to speciate. Do you mean Pseudomonas > fluorescens or fluorescence of Ps. aeruginosa? There's a BIG difference > in what you're asking. > > Judy Dilworth, M.T. (ASCP) > Microbiology > > "ALIREZA BEDELTAVANA" wrote in message > > news:mailman.190.1192302860.23109.microbio@net.bio.net... > > > > > > > Dear Sir > > I am working on the contamination of Milk with Gram-negative > > psychrotrophic spoilage bacteria and I should diagnose > > and differentiate different species of this bacteria.- Hide quoted text - > > - Show quoted text - Pseudonomas fluroscens or fluroscence from pseudomonas? From skinnerd from gmail.com Tue Nov 13 12:38:19 2007 From: skinnerd from gmail.com (skinnerd@gmail.com) Date: Tue Nov 13 17:36:45 2007 Subject: [Microbiology] Re: Complete newbie question - Texas Red In-Reply-To: References: <1194788927.632243.287050@o38g2000hse.googlegroups.com> Message-ID: <1194975499.767326.278060@v2g2000hsf.googlegroups.com> On Nov 12, 2:38 am, Bob wrote: > Check out the web site for Molecular Probes (Invitrogen), which sells > it. Look around. You may want to talk with a tech rep there. They can > discuss your specific need and suggest options. Thanks - I'll check it out. From txl8326 from louisiana.edu Wed Nov 14 15:04:03 2007 From: txl8326 from louisiana.edu (Luzan Tatiana) Date: Wed Nov 14 16:00:52 2007 Subject: [Microbiology] Problem with cultivation on anaerobic solid media Message-ID: <20071114195842.M68778@louisiana.edu> In my lab we use hungate bottles only for the liquid anaerobic media and bi- layer solid media. We have to do inoculations on solid meida only using Petri dishes in the anaerobic jar supplemented with gas-packs or controlled atmosphere anaerobic chamber. THere is a problem in the chamber: the cag mixture we use is 10% Hydrogen, 15% CO2 and Nitrogen as the rest. While incubating plates inside, the media dries up very quickly and an enourmous condensation is forming! IT is almost a lake of water there. IS there any product which would absorb an acess condensate? Thanks a lot -- Tanya Luzan Research Assistant Department of Biology University of Louisiana at Lafayette Phone (Office): 482-5056 From txl8326 from louisiana.edu Wed Nov 14 15:05:51 2007 From: txl8326 from louisiana.edu (Luzan Tatiana) Date: Wed Nov 14 16:00:58 2007 Subject: [Microbiology] hungate tube spinner Message-ID: <20071114200515.M70292@louisiana.edu> Does anybody knows where can I get an original hungate tube spinner? The bellco glass company discontinued the item. Thanks -- Tanya Luzan Research Assistant Department of Biology University of Louisiana at Lafayette Phone (Office): 482-5056 From limbic_lesion from hotmail.com Wed Nov 14 18:29:39 2007 From: limbic_lesion from hotmail.com (N10) Date: Wed Nov 14 18:57:18 2007 Subject: [Microbiology] Re: Problem with cultivation on anaerobic solid media References: Message-ID: <3-GdnTkpLZMbGabanZ2dnUVZ8sSrnZ2d@bt.com> "Luzan Tatiana" wrote in message news:mailman.615.1195074105.23109.microbio@net.bio.net... > In my lab we use hungate bottles only for the liquid anaerobic media and > bi- > layer solid media. We have to do inoculations on solid meida only using > Petri dishes in the anaerobic jar supplemented with gas-packs or > controlled > atmosphere anaerobic chamber. THere is a problem in the chamber: the cag > mixture we use is 10% Hydrogen, 15% CO2 and Nitrogen as the rest. While > incubating plates inside, the media dries up very quickly and an enourmous > condensation is forming! IT is almost a lake of water there. > > IS there any product which would absorb an acess condensate? > Thanks a lot > > -- > > Tanya Luzan > Research Assistant > Department of Biology > University of Louisiana at Lafayette > Phone (Office): 482-5056 > Hi Luzan How long are you incubating for ? I use a similar system and have no problems for up to 48 hours at 37'c You could try a Petri dish containg a layer of Silica gel ( dried) to absorb the humidity. Obvioulsy check for zero effects on colony yield :) Best Des From abhi_shri76 from yahoo.co.in Mon Nov 19 09:45:46 2007 From: abhi_shri76 from yahoo.co.in (abhinav shrivastava) Date: Mon Nov 19 14:23:24 2007 Subject: [Microbiology] Fluroscent tagging Message-ID: <339593.81505.qm@web8322.mail.in.yahoo.com> Do you have any protocol or idea regarding attachment of fluorescein with asparagine. Thanks in advance With best regards Abhinav Shrivastav Asst. Professor Biotechnology Cancer Hospital & Research Institute Gwalior (M.P.) 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From jorge1907 from aol.com Fri Nov 23 21:09:44 2007 From: jorge1907 from aol.com (Jorge) Date: Fri Nov 23 23:25:34 2007 Subject: Fwd: FW: [Microbiology] A set of tests for Pseudomonas florescence References: Message-ID: On Nov 12, 3:05 am, ALIREZA BEDELTAVANA wrote: > Dear Sir > I am working on the contamination of Milk with Gram-negative > psychrotrophic spoilage bacteria and I should diagnose > and differentiate different species of this bacteria. > I can not use Commercial kit (like API 20 NE )and I have to > make a smaller set of biochemical tests by myself. > Because some of these biochemical tests (Like in API 20 NE) > may not be necessary to diagnose this microorganism, specially > pseudomonas florescence I prefer the set be smaller > and more convenient. > Any help would be GREATLY appreciated! > > Best Regards > Alireza Bedeltavana > alirezabedeltav...@yahoo.com > > Note: forwarded message attached. > > --------------------------------- > Yahoo! Answers - Get better answers from someone who knows. Tryit now. API was developed with clinical isolates and was validated and verified 20 ways to sunday. Even in this, it is not always s accurate for id of environmental isolates. Please don't consider identification a trivial exercise of combining a small set of simple tests. From bactitech from nospamhortonsbay.com Sat Nov 24 00:44:25 2007 From: bactitech from nospamhortonsbay.com (JEDilworth) Date: Sat Nov 24 13:17:57 2007 Subject: Fwd: FW: [Microbiology] A set of tests for Pseudomonas florescence References: Message-ID: Identification of nonfermenters is not the easiest thing in the world. The API 20 NE has been around for a long time. Jorge makes a good point in that this kit is for clinical isolates and not necessarily environmental isolates. It doesn't always work that well on clinical isolates. We use is as a backup for our Vitek Legacy, although we just went live with Vitek2. It remains to be seen whether V2 does a better job with this group than Vitek Legacy. These are not necessarily a group of organisms that lend themselves to a couple of identification tests. Judy Dilworth, M.T.(ASCP) Microbiology "Jorge" wrote in message news:aaf3dff7-16e2-4551-8d53-4ee4b9e2cc86@f3g2000hsg.googlegroups.com... > > API was developed with clinical isolates and was validated and > verified 20 ways to sunday. Even in this, it is not always s accurate > for id of environmental isolates. Please don't consider > identification a trivial exercise of combining a small set of simple > tests.