[Microbiology] Re: Problem with cultivation on anaerobic solid media
(by limbic_lesion from hotmail.com)
Wed Nov 14 18:29:39 EST 2007
"Luzan Tatiana" <txl8326 from louisiana.edu> wrote in message
news:mailman.615.1195074105.23109.microbio from net.bio.net...
> In my lab we use hungate bottles only for the liquid anaerobic media and
> layer solid media. We have to do inoculations on solid meida only using
> Petri dishes in the anaerobic jar supplemented with gas-packs or
> atmosphere anaerobic chamber. THere is a problem in the chamber: the cag
> mixture we use is 10% Hydrogen, 15% CO2 and Nitrogen as the rest. While
> incubating plates inside, the media dries up very quickly and an enourmous
> condensation is forming! IT is almost a lake of water there.
> IS there any product which would absorb an acess condensate?
> Thanks a lot
> Tanya Luzan
> Research Assistant
> Department of Biology
> University of Louisiana at Lafayette
> Phone (Office): 482-5056
How long are you incubating for ? I use a similar system and have no
problems for up to 48 hours at 37'c
You could try a Petri dish containg a layer of Silica gel ( dried) to
absorb the humidity.
Obvioulsy check for zero effects on colony yield :)
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