[Microbiology] gram stain artefact

brook.clinton from csiro.au via microbio%40net.bio.net (by brook.clinton from csiro.au)
Mon Oct 8 01:04:56 EST 2007


Has anyone else seen this particular gram stain artefact?  When gram
staining Bacillus cultures i see strange light pink filaments, too
narrow to be cells, closely associated with the actual cells.  The
smears also show the characteristic gram variable reaction of
bacillus, in that the aging, endospore cells are pink, not purple.  I
don't know if these phenomena are related.  Sometimes the filamentous
stuff even looks golden rather than pink, or somewhere in between.



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