[Microbiology] Re: Suggestion for carrying out experiments with the genes involved in alkalophily of Bacillus sp.

shrinivas Dengeti via microbio%40net.bio.net (by vasu.nanobiotech91 from gmail.com)
Mon Aug 11 10:20:09 EST 2008


On Aug 9, 7:03 am, Bob <bbx107.... from excite.XYZ.com> wrote:
> On Fri, 8 Aug 2008 02:09:29 -0700 (PDT), shrinivas Dengeti
>
> <vasu.nanobiotec... from gmail.com> wrote:
> >I have planned to carry out characterization of genes involved in
> >alkalophilic nature of thermoalkalophilic Bacillus sp.
> >Somebody can suggest me some of the methodology involved in PCR
> >amplification of the genes or preliminary experiments required to
> >understand molecular basis behind the nature.
>
> What do you already know? Do you have a genome sequence? Do you know
> genes of interest? What do you know about them? Predicted protein
> sequences often offer clues about function. Do you have an expression
> system for Bacillus genes?
>
> bob

i have different gene sequence of the xylanase from the Bacillus sp.
and i have PCR amplified the gene..but not yet sequenced.
I want to know whether tranport protein play any role in the
thermoalkalophilic nature or anyother factor responsible for it....if
anyone workin on the aspect.please help me in this matter...

with regards
D.shrinivas




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