[Microbiology] Re: Suggestion for carrying out experiments with the
genes involved in alkalophily of Bacillus sp.
(by vasu.nanobiotech91 from gmail.com)
Mon Aug 11 10:20:09 EST 2008
On Aug 9, 7:03 am, Bob <bbx107.... from excite.XYZ.com> wrote:
> On Fri, 8 Aug 2008 02:09:29 -0700 (PDT), shrinivas Dengeti
> <vasu.nanobiotec... from gmail.com> wrote:
> >I have planned to carry out characterization of genes involved in
> >alkalophilic nature of thermoalkalophilic Bacillus sp.
> >Somebody can suggest me some of the methodology involved in PCR
> >amplification of the genes or preliminary experiments required to
> >understand molecular basis behind the nature.
> What do you already know? Do you have a genome sequence? Do you know
> genes of interest? What do you know about them? Predicted protein
> sequences often offer clues about function. Do you have an expression
> system for Bacillus genes?
i have different gene sequence of the xylanase from the Bacillus sp.
and i have PCR amplified the gene..but not yet sequenced.
I want to know whether tranport protein play any role in the
thermoalkalophilic nature or anyother factor responsible for it....if
anyone workin on the aspect.please help me in this matter...
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