[Microbiology] Re: Suggestion for carrying out experiments with the genes involved in alkalophily of Bacillus sp.

shrinivas Dengeti via microbio%40net.bio.net (by vasu.nanobiotech91 from gmail.com)
Sat Aug 16 07:31:47 EST 2008


thank you
bob..
for showing such a interest in helpin me out.
n thank you for the link too.
hey u still didnt got me..actually to say..i want to know whether the
transport involved in maintaining the alkalophilic nature of bacillus
for ex. ATPase , Na/H antiporyter pump,.. and one more thing do they
play any role in expresion of the enzyme
thank yoyu
With regards.
D.shrinivas

Bob wrote:

> On Mon, 11 Aug 2008 08:20:09 -0700 (PDT), shrinivas Dengeti
> <vasu.nanobiotech91 from gmail.com> wrote:
>
> >
> >i have different gene sequence of the xylanase from the Bacillus sp.
> >and i have PCR amplified the gene..but not yet sequenced.
> >I want to know whether tranport protein play any role in the
> >thermoalkalophilic nature or anyother factor responsible for it....if
> >anyone workin on the aspect.please help me in this matter...
> >
>
> A couple of quick thoughts. You've probably thought of these points
> yourself, so I�ll be brief.
>
> It is interesting to have an extracellular enzyme from an alkalophile.
> One can compare its properties with a "normal" relative, and then look
> for an explanation in the 3D structure. Do you have a good 3D
> structure for any of the related enzymes?
>
> Of course, the general alkalophilic nature of the bug probably lies
> largely in keeping the cytoplasm near "normal".
>
>
> Do you know about the following papers:
>
> http://springerlink.metapress.com/content/h3m1703x3711215t/?p=1bd8409162894aaa99d979a1fe61676d&pi=5
>
> http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6V24-4JRM055-1&_user=4420&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=4420&md5=6c95e774252e769ea34297f1bc792e49
>
> I bet you already have them :-) -- but figured it safer to ask, to be
> sure. If you don't have access, feel free to email me for copies, at
> my yahoo acct: b_bruner
>
> regards,
>
> bob


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