From baktheeswaran from gmail.com Mon Jan 7 09:14:17 2008 From: baktheeswaran from gmail.com (beswa) Date: Mon Jan 7 10:02:26 2008 Subject: [Microbiology] Microbiology Students portal Message-ID: Newly Launched Microbiology Students.com is a Exculsive information portal for Biological Science students,started and in service since 2007.Today worldover there are millions of students studying in the field of Biological Science. This website is designed for School/UG/PG/Research Students Who are in Biological Science stream. Our Portal is to provide Students to access easily the information and resources from Worldwide - all the info needed,in one place. Microbiology Students.com designed to bring useful and interesting microbiology informational resources to you. With literally billions of web pages out there in Web World, searching effectively and efficiently for any information is becoming increasingly difficult in finding accurate and specific information on microbiology subjects. This web site attempts to help you filter through the information in an organized manner. From e.bird from napier.ac.uk Tue Jan 8 10:58:29 2008 From: e.bird from napier.ac.uk (Bird, Eve) Date: Tue Jan 8 12:32:59 2008 Subject: [Microbiology] Thiamine in M9 minimal medium Message-ID: <3CCAE204656C9D4D91C118CD01EF63D004E76B71@EVS2.napier-mail.napier.ac.uk> Hello, I noticed you posts while I was desperately trying to find a reason why my top 10 transformants would not grow in M9 media. Which recipes worked for you in the end? Many thanks, Eve Bird This message is intended for the addressee(s) only and should not be read, copied or disclosed to anyone else outwith the University without the permission of the sender. It is your responsibility to ensure that this message and any attachments are scanned for viruses or other defects. Napier University does not accept liability for any loss or damage which may result from this email or any attachment, or for errors or omissions arising after it was sent. Email is not a secure medium. Email entering the University's system is subject to routine monitoring and filtering by the University. From deepgolwala from gmail.com Tue Jan 8 11:35:30 2008 From: deepgolwala from gmail.com (deepgolwala@gmail.com) Date: Tue Jan 8 12:33:03 2008 Subject: [Microbiology] media preparations Message-ID: <6e375f8a-94f5-427f-bd03-6ed7f7789007@v4g2000hsf.googlegroups.com> i am new to the field , i would like to ask while making LB agar(tryptone-10 gms,yeast extract-5 gms,NaCl-10 gms-all weight for 1000ml preparations)when should i add agar during preparations or after autoclaving. From farrlarr from isu.edu Tue Jan 8 12:25:56 2008 From: farrlarr from isu.edu (Larry Farrell) Date: Tue Jan 8 23:57:43 2008 Subject: [Microbiology] Re: media preparations In-Reply-To: <6e375f8a-94f5-427f-bd03-6ed7f7789007@v4g2000hsf.googlegroups.com> References: <6e375f8a-94f5-427f-bd03-6ed7f7789007@v4g2000hsf.googlegroups.com> Message-ID: <4783a67f$0$26030$88260bb3@free.teranews.com> deepgolwala@gmail.com wrote: > i am new to the field , > i would like to ask while making LB agar(tryptone-10 gms,yeast > extract-5 gms,NaCl-10 gms-all weight for 1000ml preparations)when > should i add agar during preparations or after autoclaving. Agar will survive autoclaving without any problems, and the heat involved will melt the agar so it can be easily distributed throughout the medium by swirling the container after it is removed from the autoclave and cooled for 10-15 minutes. As the medium cools sufficiently for pouring of plates, most/all of the bubbles introduced by the mixing will spontaneously pop so there will be few if any left when the plates are poured. If you wait until the agar is cool enough to pour to mix, all of those bubbles will be left and you will have to deal with them by flaming the surfaces of virtually all the plates you pour. A question that you might want to consider is how you would prepare sterile agar for addition to medium that has already been autoclaved. There really isn't any convenient way to do that, to my knowledge. Even if there is such a method, the agar solution would have to be heated sufficiently to melt the agar, which pretty much boils down, again, to autoclaving. -- Larry D. Farrell, Ph.D. Professor of Microbiology Idaho State University -- Posted via a free Usenet account from http://www.teranews.com From GreenieLeBrun from hotmail.com Tue Jan 8 16:40:59 2008 From: GreenieLeBrun from hotmail.com (GreenieLeBrun) Date: Tue Jan 8 23:57:50 2008 Subject: [Microbiology] Re: media preparations References: <6e375f8a-94f5-427f-bd03-6ed7f7789007@v4g2000hsf.googlegroups.com> Message-ID: deepgolwala@gmail.com wrote: > i am new to the field , > i would like to ask while making LB agar(tryptone-10 gms,yeast > extract-5 gms,NaCl-10 gms-all weight for 1000ml preparations)when > should i add agar during preparations or after autoclaving. If you are so new to the field that you need to ask a question such as that you need to do some reading. Two hints, 1. agar powder is not sterile. 2. agar needs to be brought to the boil to dissolve it. From yjgent from nospamcox.net Tue Jan 8 21:00:05 2008 From: yjgent from nospamcox.net (John Gentile) Date: Tue Jan 8 23:58:12 2008 Subject: [Microbiology] Re: media preparations References: <6e375f8a-94f5-427f-bd03-6ed7f7789007@v4g2000hsf.googlegroups.com> Message-ID: <2008010821000516807-yjgent@nospamcoxnet> On 2008-01-08 11:35:30 -0500, deepgolwala@gmail.com said: > i am new to the field , > i would like to ask while making LB agar(tryptone-10 gms,yeast > extract-5 gms,NaCl-10 gms-all weight for 1000ml preparations)when > should i add agar during preparations or after autoclaving. Making media is one of the things I truely enjoyed in my early days in the micro lab. With experience you will pick up a lot of good tips on making quality media - usually better than any factory made! A few points I can pass along, - agar melts around 96 C, and solidifies around 45-50C. so make sure you add the agar to your mix and boil it - but watch for over boiling! Then autoclave it in a round flask to avoid boilover (Erlenmeyer Flasks and "Fleakers" are known for this). Don't seal the flask tight, but use a gauze plug in the mouth to allow steam and pressure to equalize. Cool the solution in a 56C waterbath and decant when cooled. I used to use a spring loaded refilable syringe to make plates and tubes, but hand pouring is pretty good too. If you get bubbles in the media you need to use a bunsen burner to flame the bubbles off before the media solidifies. - some media contains bile salts that precipitate out with heat. These I heated only to melt the agar and poured immediately. XLD is an example, and did not need autoclaving. - some media have added ingredients that cannot be boiled or autoclaved. These need to be filter sterilized and then added after the autoclaved media has cooled and then can be decanted. Get a good media book that contains recipies and also preparation tips. I always used Difco and BD's book, but I don't know what is out there now! -- John Gentile MS, M(ASCP) Laboratory Information Mgr. VA Medical Center Providence, RI yjgent@cox.net From tcaetano from ua.pt Thu Jan 10 14:30:23 2008 From: tcaetano from ua.pt (=?ISO-8859-1?Q?T=E2nia_Caetano?=) Date: Thu Jan 10 15:30:31 2008 Subject: [Microbiology] high molecular plasmid DNA Message-ID: <20F765B1-A0C3-439A-A19E-DB3CE17C53AA@ua.pt> Hello everyone Does anyone know why in some cases we see (in an 0,8% agarose gel) plasmid DNA higher than chromosomal DNA? I know that there are very large plasmids but it is possible to exist a plasmid bigger than the chromosome? I thought it wasn't possible. I thought that is a characteristic of the separation of the agarose gels but if this is the case, how can we explain it? Looking forward to hear from you something Best wishes bio From hotbacteria from rediffmail.com Fri Jan 11 14:24:50 2008 From: hotbacteria from rediffmail.com (tarun gupta) Date: Fri Jan 11 15:15:46 2008 Subject: [Microbiology] Re: high molecular plasmid DNA Message-ID: <20080111192450.14499.qmail@f5mail-237-202.rediffmail.com> ? Hi, This sounds strange; unless your Chromosomal DNA is fragmented, I don't see how can it even move further through the wells! Kind Regards, Tarun Gupta -- MSc Human Genomics National Centre for Human Genome Studies and Research Panjab University Chandigarh-India Appeal: Let's not make information inaccessible to masses; Let's publish in Open Access Journals! ************************************************* ~Exciting Science forum: http://groups.google.com/group/exciting-science ~About Me: http://hotbacteria.blogspot.com ~Scientific Information Sharing Resource: http://sisr.blogspot.com ~My Department: http://nchgsr.puchd.ac.in ************************************************* T?nia Wrote: > >Hello everyone > >Does anyone know why in some cases we see (in an 0,8% agarose gel) >plasmid DNA higher than chromosomal DNA? >I know that there are very large plasmids but it is possible to exist >a plasmid bigger than the chromosome? I thought it wasn't possible. >I thought that is a characteristic of the separation of the agarose >gels but if this is the case, how can we explain it? > >Looking forward to hear from you something >Best wishes >bio MSc Human Genomics National Centre of Human Genome Studies and Research Panjab University Chandigarh-India Mob: +91-9888237906 ************************************************* ~Exciting Science forum: http://groups.google.com/group/exciting-science ~Bioinformatics Resources: http://hotbacteria.blogspot.com ~Scientific Information Sharing Resource: http://sisr.blogspot.com ~About Me: http://hotbacteria.tripod.com ************************************************* From biotecprocess from gmail.com Mon Jan 14 02:34:18 2008 From: biotecprocess from gmail.com (Shlomo Pleban) Date: Mon Jan 14 12:55:55 2008 Subject: [Microbiology] Lipase Message-ID: <5ec09cd60801132334j901df43tb51f907386065065@mail.gmail.com> Hi, Does someone has method to detect lipase activity in Acrylamid gel Thank you all in advance Shlomo From devishyamala.s from gmail.com Wed Jan 16 05:04:57 2008 From: devishyamala.s from gmail.com (devishyamala.s@gmail.com) Date: Wed Jan 16 12:22:52 2008 Subject: [Microbiology] amylase production Message-ID: <5c04457a-37b2-4534-b147-b70d248434e6@e25g2000prg.googlegroups.com> hi all.... i'm doing project in production of amylase from waste . is there any reference for amylase production from paddy straw by A.niger. anybody help me.. From carol.smee from prolysis.com Thu Jan 24 09:50:02 2008 From: carol.smee from prolysis.com (Carol Smee) Date: Thu Jan 24 12:07:13 2008 Subject: [Microbiology] Origin of E. coli DC2 [Scanned] Message-ID: <3E9173D1900758458965B5A9D501CAD203D9D0@PRO-PC-01.prolysis.local> Antimicrob Agents Chemother. 1976 August; 10(2): 215-218 Richmond, Clark and Wotton (1976) :-) Carol Smee Senior Scientist Prolysis Ltd. Unit 6, Begbroke Science Park Sandy Lane, Yarnton Oxfordshire. OX5 1PF, UK +44 (0) 1865 854700 www.prolysis.com From seeseemeagain from hotmail.com Thu Jan 24 17:58:05 2008 From: seeseemeagain from hotmail.com (newsnews) Date: Thu Jan 24 19:21:36 2008 Subject: [Microbiology] help request Message-ID: hi, if you have some BioRad SYBR Green Supermix i can borrow, please kindly let me know. i need it urgently. xqiu@purdue.edu. thanks From Ante.Krstulovic from sanofi-aventis.com Wed Jan 30 10:49:31 2008 From: Ante.Krstulovic from sanofi-aventis.com (Ante.Krstulovic@sanofi-aventis.com) Date: Wed Jan 30 15:30:33 2008 Subject: [Microbiology] Rapid identification of Micro-organisms using Mass Spectrometry. Message-ID: Dear Dr. Smith, I would like to know if you would be interested in giving a lecture on the identification of microorganisms at ourCompany. I am looking forward to your reply. With kind regards, Ante Krstulovic, Ph.D. Analytical Sciences Department Sanofi-Aventis