[Microbiology] Re: media preparations

GreenieLeBrun via microbio%40net.bio.net (by GreenieLeBrun from hotmail.com)
Tue Jan 8 16:40:59 EST 2008



deepgolwala from gmail.com wrote:
> i am new to the field ,
> i would like to ask while making LB agar(tryptone-10 gms,yeast
> extract-5 gms,NaCl-10 gms-all weight for 1000ml preparations)when
> should i add agar during preparations or after autoclaving.

If you are so new to the field that you need to ask a question such as that 
you need to do some reading.

Two hints,

1. agar powder is not sterile.
2. agar needs to be brought to the boil to dissolve it.




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