[Microbiology] Re: media preparations
(by GreenieLeBrun from hotmail.com)
Tue Jan 8 16:40:59 EST 2008
deepgolwala from gmail.com wrote:
> i am new to the field ,
> i would like to ask while making LB agar(tryptone-10 gms,yeast
> extract-5 gms,NaCl-10 gms-all weight for 1000ml preparations)when
> should i add agar during preparations or after autoclaving.
If you are so new to the field that you need to ask a question such as that
you need to do some reading.
1. agar powder is not sterile.
2. agar needs to be brought to the boil to dissolve it.
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