[Microbiology] Re: Reference
(by yjgent from nospamcox.net)
Sat May 17 20:26:54 EST 2008
On 2008-05-16 16:04:25 -0400, senaatlaw Atlaw Woldeyohanes
<senaatlaw from yahoo.com> said:
> Dear Sir,
> I have isolated a Microorganism, Gram-negative Coccobacillus
> Streaked it on MacConkey agar, pink colonies started to grow before
> eight hours.
> Today after 24 hrs, it is Lactose fermenting, good pink colonies,
> and is Oxidase positive too, I thought it would be from
> Enterobactericeae, but it is rare to be Oxidase positive or unless one
> of the rare ?
> I only have the basic id. Material, and I am going to send it for
> identification to another Lab.
> But I am so curious what can it be, I try to find out on my
> Diagnostic Microbiol. Book, nothing matches at this moment I may have
> to see more.
> Mac, Positive and Oxidase positive, if there is some common
> microorganism which matches this or a good reference, would you please
> let me know.
We're going to need a bit more information. Is this a human isolate,
animal or environmental? Did you do basic id media like TSI, Urea,
motility, Indole? Are you sure it is a pure isolate?
Make sure you streak for isolation, select a single colony. Is your
oxidase reagent fresh?
I know of only one human isolate that could (<3%) be lactose pos and
oxidase pos. Also be careful about your Gram's stain morphology.
John Gentile MS, M(ASCP)
Laboratory Information Mgr.
VA Medical Center
yjgent from cox.net
More information about the Microbio