[Microbiology] Re: extracting compounds from agar plates

N10 via microbio%40net.bio.net (by limbic_lesion from hotmail.com)
Mon May 19 16:52:00 EST 2008


"Pep Charusanti" <pcharusa from bioeng.ucsd.edu> wrote in message 
news:mailman.134.1211223442.3533.microbio from net.bio.net...
> Hello everybody,
>
> Does anybody have a protocol on how to extract compounds from agar?  I am 
> growing bacteria on plates and they are secreting compounds (probably 
> metabolites) into the agar that I would like to isolate.  I tried growth 
> in liquid culture, but by HPLC analysis of the supernatant I know this 
> does not lead to production of the compounds I'm interested in.
>


Hello Pep

What group of compounds are you interested in ?

regards N10




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