[Microbiology] Re: membrane filtration technique

N10 via microbio%40net.bio.net (by limbic_lesion from hotmail.com)
Mon Nov 24 19:17:27 EST 2008


<monarch00 from comcast.net> wrote in message 
news:db9ki4ph7ffflcftg858kf7lg5licjaf1l from 4ax.com...
> What are the rules for counting and calculating for total bacteria
> using membrane filtration technique?


Heloo


Re counting ;the  colony count  per ml is returned as colonies/volume 
filtered mls

Obviously the lowest colony counts is  0 which rightly would be expressed as 
< 1 colony  per volume filtered

In the case of low numbers you would correcftly express the count obtained 
with some measure of uncertainty  of measurmemnt which is usually  the
count obtained  +/- the sd*1.96  for lower range counts.

There will be upper limit to the number of colonies you can count on a 
membrane  but that will depend on which protcol you are using and the size 
of filter; perhapes another can help on this one.

Best N10







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