[Microbiology] Re: membrane filtration technique
(by limbic_lesion from hotmail.com)
Mon Nov 24 19:17:27 EST 2008
<monarch00 from comcast.net> wrote in message
news:db9ki4ph7ffflcftg858kf7lg5licjaf1l from 4ax.com...
> What are the rules for counting and calculating for total bacteria
> using membrane filtration technique?
Re counting ;the colony count per ml is returned as colonies/volume
Obviously the lowest colony counts is 0 which rightly would be expressed as
< 1 colony per volume filtered
In the case of low numbers you would correcftly express the count obtained
with some measure of uncertainty of measurmemnt which is usually the
count obtained +/- the sd*1.96 for lower range counts.
There will be upper limit to the number of colonies you can count on a
membrane but that will depend on which protcol you are using and the size
of filter; perhapes another can help on this one.
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