[Microbiology] Counting bacteria
(by ruthsiele from yahoo.com)
Sat Oct 18 10:54:12 EST 2008
Thanks in advance for your help.
I have bacteria and a membrane
My objective is to analyze the deposition of the bacteria on the membrane with time.
To do this, - I have prepared 0.1 OD (600) bacterial solution
- I run the solution through the membrane in a flow cell under flourescent
- I took pictures of the deposited bacteria in every 15 minutes using 40x
*Now, i want to Aanlyze the number of bacteria deposited on the membrane with time.
* Thus, instead of counting all the deposited bacteria one by one, I am looking for any
othere means which can help me get the number of deposited bacteria per unit area of
the membrane? I was using the 40x lens.
Thanks so much again for your help,
looking forward to hearing from you
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