From linasmuch from gmail.com Thu Sep 4 11:54:57 2008 From: linasmuch from gmail.com (Lin Andis) Date: Thu Sep 4 17:21:51 2008 Subject: [Microbiology] questions about a virus Message-ID: <3fad3f010809040954i75c0027bt972d7559d4521b3f@mail.gmail.com> I am confused maybe you can help me sort it out. Has medical science ever found a "cure" for a virus? Any virus? I know we have vaccines for them=85 at least I think we do=85 isn't polio and smallpo= x a virus. What about chicken-pox and measles and all those childhood diseases that we get only once? We are "vaccinated" by getting the virus and creating our own antibodies (like the article said). Right? And does all o= f this mean that we will probably not find a "cure" but rather a "vaccine" fo= r Aids? I hope I haven't ask too many questions, and hope I am email the right person, thanks in advance for they help=85 if you aren't the person that I = am looking for; maybe you could put me in touch with someone that could answer my questions. Inquiringly, Lin linasmuch@gmail.com I found your email on this website http://iubio.bio.indiana.edu/biomail/listinfo/microbio From limbic_lesion from hotmail.com Thu Sep 4 17:46:18 2008 From: limbic_lesion from hotmail.com (N10) Date: Fri Sep 5 10:31:37 2008 Subject: [Microbiology] Re: questions about a virus References: Message-ID: Lin Hello What a really good question....well done In broad brush strikes what you assert is in the main true. Most treatments for viral diseases are preventative via vaccination route. Alternatively in a relative small number of cases medications may be used to control or suppress dromal diseases. Example include control of HIV, Herpes type I and some forms of Hepatitis. Conditions caused by ocogenic viruses tend to focus relatively destructive regimes targeted at infected tissues. Why are you interested ? N10 "Lin Andis" wrote in message news:mailman.1382.1220566911.3533.microbio@net.bio.net... I am confused maybe you can help me sort it out. Has medical science ever found a "cure" for a virus? Any virus? I know we have vaccines for them… at least I think we do… isn't polio and smallpox a virus. What about chicken-pox and measles and all those childhood diseases that we get only once? We are "vaccinated" by getting the virus and creating our own antibodies (like the article said). Right? And does all of this mean that we will probably not find a "cure" but rather a "vaccine" for Aids? I hope I haven't ask too many questions, and hope I am email the right person, thanks in advance for they help… if you aren't the person that I am looking for; maybe you could put me in touch with someone that could answer my questions. Inquiringly, Lin linasmuch@gmail.com I found your email on this website http://iubio.bio.indiana.edu/biomail/listinfo/microbio From simmel from its.jnj.com Thu Sep 11 03:49:36 2008 From: simmel from its.jnj.com (Immelman, Sandra [CONZA]) Date: Thu Sep 11 16:39:29 2008 Subject: [Microbiology] Micobes in a bar of soap Message-ID: <4690A56A1919EC4DAE9D8773203918CF017950A2@JNJZAELGMS01.eu.jnj.com> We are looking for a test method that can determine the total count of colonies INSIDE a bar of soap. We would like to obtain a method where we can make a dilution in a broth and then follow a spread plate technique to count the final colonies. We are however not sure how to go about this ex dilution factor etc. Any ideas or suggestions? From jorge1907 from aol.com Fri Sep 12 16:52:27 2008 From: jorge1907 from aol.com (jorge1907@aol.com) Date: Fri Sep 12 20:41:58 2008 Subject: [Microbiology] Re: Microbio Digest, Vol 40, Issue 2 In-Reply-To: <200809121706.m8CH6UV18507@net.bio.net> References: <200809121706.m8CH6UV18507@net.bio.net> Message-ID: <8CAE338FB93424D-BE0-237E@Webmail-mg15.sim.aol.com> You need to consider that microbes surviving?"inside of a bar soap" may not be detected using typical dilution and typical agar plating methods.? Curious, why would you think microbes would be present inside a bar of soap?? Soap making is a pretty harsh process. -----Original Message----- From: microbio-request@oat.bio.indiana.edu To: microbio@magpie.bio.indiana.edu Sent: Fri, 12 Sep 2008 1:06 pm Subject: Microbio Digest, Vol 40, Issue 2 Send Microbio mailing list submissions to microbio@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/microbio or, via email, send a message with subject or body 'help' to microbio-request@net.bio.net You can reach the person managing the list at microbio-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Microbio digest..." Today's Topics: 1. Micobes in a bar of soap (Immelman, Sandra [CONZA]) 2. Re: Micobes in a bar of soap (Bob) ---------------------------------------------------------------------- Message: 1 Date: Thu, 11 Sep 2008 10:49:36 +0200 From: "Immelman, Sandra [CONZA]" Subject: [Microbiology] Micobes in a bar of soap To: Message-ID: <4690A56A1919EC4DAE9D8773203918CF017950A2@JNJZAELGMS01.eu.jnj.com> Content-Type: text/plain; charset="iso-8859-1" We are looking for a test method that can determine the total count of colonies INSIDE a bar of soap. We would like to obtain a method where we can make a dilution in a broth and then follow a spread plate technique to count the final colonies. We are however not sure how to go about this ex dilution factor etc. Any ideas or suggestions? ------------------------------ Message: 2 Date: Thu, 11 Sep 2008 21:25:16 -0700 From: Bob Subject: [Microbiology] Re: Micobes in a bar of soap To: microbio@net.bio.net Message-ID: Content-Type: text/plain; charset=us-ascii On Thu, 11 Sep 2008 10:49:36 +0200, "Immelman, Sandra [CONZA]" wrote: >We are looking for a test method that can determine the total count of colonies INSIDE a bar of soap. We would like to obtain a method where we can make a dilution in a broth and then follow a spread plate technique to count the final colonies. We are however not sure how to go about this ex dilution factor etc. Any ideas or sug gestions? You want to express the final result as CFU per gram of soap. That means you must know the mass of soap and vol of liquid that you use for the first step, of suspending the soap. You'll have to worry about how to get the bugs off the soap, and any possible effects of the soap on the plating. You can explore these with controls. bob ------------------------------ _______________________________________________ Microbio mailing list Microbio@net.bio.net http://www.bio.net/biomail/listinfo/microbio End of Microbio Digest, Vol 40, Issue 2 *************************************** From vaanie_84 from yahoo.com Wed Sep 17 23:27:10 2008 From: vaanie_84 from yahoo.com (litnes) Date: Thu Sep 18 11:50:40 2008 Subject: [Microbiology] pH 3 Glucose yeast peptone agar medium does not solidified.... Message-ID: <58abcd9a-294c-4f8b-b660-38621886e04d@a8g2000prf.googlegroups.com> Good afternoon everyone, I have a difficulity in solidifying the agar medium to pH 3. the agar that i used is glucose yeast peptone, the composition is as follows, 1. glucose - 5g 2. peptone - 5g 3. Yeast extract - 3g 4. Agar - 20g 5. Distilled water - 1000ml The agar was prepared as usual, but the pH was changed into a range, from pH 3 to pH 7. After autoclaving the media, the other medium with different range of pH is able to solidified, but the pH 3 does not solidified at all. It remain as a broth even the agar was added before this. In addition, the pH 4 medium is partially solidified, that means it shows the semi solid characteristic. I have repeated the preparation but it still give me the same result. I do not know what parameter that i have to change it now. Is i need to change the composition agar into much higher percentage? From M.F.Yuan from gmail.com Sat Sep 20 06:36:28 2008 From: M.F.Yuan from gmail.com (Menglong F. Yuan) Date: Sat Sep 20 12:50:37 2008 Subject: [Microbiology] Re: pH 3 Glucose yeast peptone agar medium does not solidified.... References: <58abcd9a-294c-4f8b-b660-38621886e04d@a8g2000prf.googlegroups.com> Message-ID: <112e358c-f934-457f-9819-e4af57c37878@w39g2000prb.googlegroups.com> On Sep 18, 12:27?pm, litnes wrote: > Good afternoon everyone, > > I have a difficulity in solidifying the agar medium to pH 3. the agar > that i used is glucose yeast peptone, the composition is as follows, > > 1. glucose - 5g > 2. peptone - 5g > 3. Yeast extract - 3g > 4. Agar - 20g > 5. Distilled water - 1000ml > > The agar was prepared as usual, but the pH was changed into a range, > from pH 3 to pH 7. After autoclaving the media, the other medium with > different range of pH is able to solidified, but the pH 3 does not > solidified at all. It remain as a broth even the agar was added before > this. In addition, the pH 4 medium is partially solidified, that means > it shows the semi solid characteristic. I have repeated the > preparation but it still give me the same result. I do not know what > parameter that i have to change it now. Is i need to change the > composition agar into much higher percentage? I've met this problem before when I using TGY agar. I don't think changing the composition agar into much higher percentage might do effects. Why don't you use the phytagel instead of agar? From ruthsiele from yahoo.com Fri Sep 19 14:22:11 2008 From: ruthsiele from yahoo.com (RUTH SIELE) Date: Sat Sep 20 12:50:41 2008 Subject: [Microbiology] isolation of bacteria Message-ID: <575505.15887.qm@web45208.mail.sp1.yahoo.com> hi all, Thanks for your suggestions in advance, I am trying to isolate the bacteria from brackish water. I incubated the brackish water for three weeks. When I centrifuge the culture after three weeks, I couldn't get any pellets to?extract the DNA.?I?streak them in LB plates but it will be culture dependent. ? Thus, I am looking for your suggestions if there is other means by which i can extract the DNA or if there is anything I have to do so as to get pellets from the culture ? Thanks, ? Ruth From bactitech from nospamhortonsbay.com Sun Sep 21 01:32:17 2008 From: bactitech from nospamhortonsbay.com (JEDilworth) Date: Sun Sep 21 13:05:39 2008 Subject: [Microbiology] Re: isolation of bacteria References: Message-ID: Three weeks of incubation for bacteria seems excessive. I know nothing about DNA extraction or environmental bacteria, but I do know that bacteria go through growth phases. Eventually they will be living in their own filth and die off. Why did you decide on three weeks? Are you inoculating into anything nutritional? Are you passing your growth into new broths along the way? Why not streak the water onto solid media and see what grows? May have to do dilutions until you find a dilution where you can get isolated colonies. Pick the colonies individually, one at a time, and put them into their own broth, and then you will have pure cultures. Why work with mixed cultures? I have no idea what media you would use for this - just putting the concept out there. I know we do water cultures from time to time and use something called Standard Methods agar. We buy the deeps commercially. We are not dealing with this type of water, however. We're culturing distilled water and checking for contaminants. What temperature are you using? I would think environmental bacteria need cooler temperatures than, say, 35 degrees C which is what we incubate human medical bacterial specimens at. Again, I am a medical micro person - NOT an environmental one. Robert Koch discovered solid media in the 1880's. This is how he got a leg up on Louis Pasteur, who never figured out how to move beyond broth cultures and work with pure cultures. Solid media is the foundation of modern bacteriology. Judy Dilworth, M.T. (ASCP) Microbiology "RUTH SIELE" wrote in message news:mailman.82.1221933057.29717.microbio@net.bio.net... I am trying to isolate the bacteria from brackish water. I incubated the brackish water for three weeks. When I centrifuge the culture after three weeks, I couldn't get any pellets to extract the DNA. I streak them in LB plates but it will be culture dependent. From axp955 from psu.edu Sun Sep 21 13:26:44 2008 From: axp955 from psu.edu (AMRITA PURI) Date: Mon Sep 22 12:38:30 2008 Subject: [Microbiology] isolation of bacteria In-Reply-To: 575505.15887.qm@web45208.mail.sp1.yahoo.com References: <575505.15887.qm@web45208.mail.sp1.yahoo.com> Message-ID: <1222021604l.323640l.0l@psu.edu> Hi I extract DNA from environmental samples like soil and compost..and also from compost suspensions...So u could give the kit that i use a try...its called the Mobio Power Soil DNA kit...It is a really good kit and works for dry and wet soil samples...so i personally feel it might work to extract DNA from your samples..Here is the link Hope this helpsA On Fri, Sep 19, 2008 03:22 PM, RUTH SIELE wrote: > hi all, >Thanks for your suggestions in advance, >I am trying to isolate the bacteria from brackish water. I incubated the >brackish water for three weeks. When I centrifuge the culture after three >weeks, I couldn't get any pellets to?extract the DNA.?I?streak them in LB >plates but it will be culture dependent. >? >Thus, I am looking for your suggestions if there is other means by which i can >extract the DNA or if there is anything I have to do so as to get pellets from >the culture >? >Thanks, >? >Ruth > > > >_______________________________________________ >Microbio mailing list >Microbio@net.bio.net >http://www.bio.net/biomail/listinfo/microbio > > > From blackhole from abuse.plus.com Mon Sep 22 08:15:13 2008 From: blackhole from abuse.plus.com (Duncan Clark) Date: Mon Sep 22 12:39:04 2008 Subject: [Microbiology] Re: isolation of bacteria References: Message-ID: Historians believe that in newspost on Fri, 19 Sep 2008, RUTH SIELE penned the following literary masterpiece: >I am trying to isolate the bacteria from brackish water. I incubated the brackish water for three weeks. When I centrifuge the culture after three >weeks, I couldn't get any pellets to?extract the DNA.?I?streak them in LB plates but it will be culture dependent. Incubate how? What makes you think the bugs there will actually grow on LB? Some might but it's probably way off the optimum for most. A chemical analysis of the water will give some insights to it's make up. Failing that, at least try simply plating on brackish water agar plates i.e. filter the water and autoclave with agar. >? >Thus, I am looking for your suggestions if there is other means by which i can extract the DNA or if there is anything I have to do so as to get >pellets from the culture Take 10-100litres of water direct from the brackish water source. Simply centrifuge or concentrate by ultra filtration. Lyse cells from whatever pellet you get (possibly using one of the soil isolation kits from Qiagen or similar) and phi29 amplify whatever DNA is there using a Genomphi kit from GE Healthcare. If you can get 1-10ng of DNA to start with you will at least get 4-5ug back after amplification. Duncan -- I love deadlines. I especially like the whooshing noise they make as they go flying by. Duncan Clark GeneSys Ltd. From omorais from estg.ipvc.pt Mon Sep 22 04:06:10 2008 From: omorais from estg.ipvc.pt (Orlando Morais) Date: Mon Sep 22 13:10:19 2008 Subject: [Microbiology] Re: pH 3 Glucose yeast peptone agar medium does not Message-ID: <005301c91c92$7508e550$5f1aaff0$@ipvc.pt> Try this Autoclave the media at normal pH. After sterilization adjust the pH with a sterile acid (lactic acid) Orlando Morais Unidade de Microbiologia Aplicada Escola Superior de Tecnologia e Gest?o Instituto Polit?cnico de Viana do Castelo Telefone: +351 258 819 700 Fax: + 351 258 827 636 http://www.estg.ipvc.pt/ From ruthsiele from yahoo.com Mon Sep 22 15:33:25 2008 From: ruthsiele from yahoo.com (RUTH SIELE) Date: Mon Sep 22 16:24:42 2008 Subject: [Microbiology] Bacterial isolation Message-ID: <403129.30772.qm@web45213.mail.sp1.yahoo.com> HI all, ? Thank so much all for your constructive suggestions and advieces, it's alot to me. As we all know, mebrane fouling is one of the main problems of the seawater and brackish water desalination plants. Thus, specifically my objective is to identify the type of bacteria in the brackish water sample that i collected. To do this: 1. I incubated 20mL of the brackish water, 30 oC 2. I added 10mL of the same brackish water and descard 10mL from the 20mL every 24 hr. 3. I kept doing this till I get turbid culture (for three weeks so far), but when I centrifuged it so that to extract the DNA, I couldn't get any pellets. 4. Thus, to check if I have any bacteria in the brackish water, I streaked them in LB plates, and I found two types of colonies (as I saw it morphologically), but there could be other bacteria in the culture which can not grow in the LB plates. This was my problem. I really thank you all for your ideas and suggestions. And I look forward for more advices. Thank you so much again, Ruth From kdevik from gmail.com Wed Sep 24 09:38:36 2008 From: kdevik from gmail.com (Kanchanadevi k) Date: Wed Sep 24 14:03:01 2008 Subject: [Microbiology] doubt about bacterial isolation Message-ID: <8b3d015d0809240738y7b5e4bfaifcb99e954697a59e@mail.gmail.com> Hi every one Thanks a lot in advance. I need to isolate bacteria from rhizosphere soil I have a following doubt in soil sample collection. so, can you please help me to make me clear Actually i need to collect a rhizosphere soil so, i have a doubt Is there any rules that we need to collect soil in particular temperature or during mansoon season like that. I think that i need to collect soil during mansoon season am i right ? please make me clear or if there is any general protocol for How to collect rhizosphere soil for bacterial isolation. tell me from where i can get that. thank u so much once again waiting for ur reply -- K.Kanchana Devi, Research Scholar, Centre for Environmental Management of Degraded Ecosystem, University of Delhi, Delhi - 110 007. India From sivaamuthum from yahoo.com Thu Sep 25 11:15:12 2008 From: sivaamuthum from yahoo.com (siva prasad) Date: Thu Sep 25 11:31:12 2008 Subject: [Microbiology] lipse project Message-ID: <938140.50756.qm@web34305.mail.mud.yahoo.com> Respected sir/Madam ?????????????????????????I?have planned to produe lipase enzyme from marine water (sea water).My works are as follows ?????? 1.? optimization of diiferent media for the growth and production(2 liter)?of lipase??enzyme ?????? 2.Optimization of pH ?????? 3.Addtion of precursor to produce more amount of?lipase enzyme ???????4.Isolation of bcteria from?sea water ?? ? can you tell the spp and genus name of the bacteria from marine?(sea water) ???????????????????? How?do identify the lipase producing bacteria in the colonies ???????????????????? what is the selective media for that bacteria ?????????????????????What is the Bichemical test to check the lipase activity?? ?I am waiting for your valuable reply ?????????????????????????????????????????? Thankingyou Siva From medanrc from yahoo.com Fri Sep 26 03:00:41 2008 From: medanrc from yahoo.com (mohamed eda) Date: Fri Sep 26 13:39:47 2008 Subject: [Microbiology] I need help in phytase activity Message-ID: <569651.44185.qm@web65514.mail.ac4.yahoo.com> I isolated some fungal isolates from compost sample and I want to measure the phytase activity of theses fungi in the liquid media. I want to ask about which media can I use and the method for measuring the phytase activity in this liquid media thanks in advance ? Mohamed Eida Agricultural Microbiology Dept. National Research Center, Egypt Hiroshima University Graduate School of Biosphere Science Assessment of Environmental Dynamics Dept. Plant Environmental Science Lab. phone No. 090-6405-4222 (SoftBank)