[Microbiology] Re: Microbio Digest, Vol 40, Issue 2

jorge1907 from aol.com via microbio%40net.bio.net (by jorge1907 from aol.com)
Fri Sep 12 16:52:27 EST 2008


You need to consider that microbes surviving?"inside of a bar soap" may not be detected using typical dilution and typical agar plating methods.? 

Curious, why would you think microbes would be present inside a bar of soap?? Soap making is a pretty harsh process.



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Subject: Microbio Digest, Vol 40, Issue 2



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Today's Topics:

   1. Micobes in a bar of soap (Immelman, Sandra [CONZA])
   2. Re: Micobes in a bar of soap (Bob)


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Message: 1
Date: Thu, 11 Sep 2008 10:49:36 +0200
From: "Immelman, Sandra [CONZA]" <simmel from its.jnj.com>
Subject: [Microbiology] Micobes in a bar of soap
To: <microbio from magpie.bio.indiana.edu>
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    <4690A56A1919EC4DAE9D8773203918CF017950A2 from JNJZAELGMS01.eu.jnj.com>
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We are looking for a test  method that can determine the total count of colonies 
INSIDE a bar of soap. We would like to obtain a method where we can make a 
dilution in a broth and then follow a spread plate technique to count the final 
colonies. We are however not sure how to go about this ex dilution factor etc. 
Any ideas or suggestions? 


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Message: 2
Date: Thu, 11 Sep 2008 21:25:16 -0700
From: Bob <bbx107.XYZ from excite.XYZ.com>
Subject: [Microbiology] Re: Micobes in a bar of soap
To: microbio from net.bio.net
Message-ID: <r3pjc4lrirupa5mupjr5h3slp4ff0u7adt from 4ax.com>
Content-Type: text/plain; charset=us-ascii

On Thu, 11 Sep 2008 10:49:36 +0200, "Immelman, Sandra [CONZA]"
<simmel from its.jnj.com> wrote:

>We are looking for a test  method that can determine the total count of 
colonies INSIDE a bar of soap. We would like to obtain a method where we can 
make a dilution in a broth and then follow a spread plate technique to count the 
final colonies. We are however not sure how to go about this ex dilution factor 
etc. Any ideas or sug
gestions? 

You want to express the final result as CFU per gram of soap. That
means you must know the mass of soap and vol of liquid that you use
for the first step, of suspending the soap.

You'll have to worry about how to get the bugs off the soap, and any
possible effects of the soap on the plating. You can explore these
with controls.

bob


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