From s02001213 from student.usp.ac.fj Wed Apr 1 00:30:52 2009 From: s02001213 from student.usp.ac.fj (s02001213@student.usp.ac.fj) Date: Wed Apr 1 12:21:28 2009 Subject: [Microbiology] re:which medium Message-ID: <1238563852.49d2fc0c332d4@webmail.student.usp.ac.fj> Hi.Please help!! Which medium do i use for the cultivation of this particular bacteria in the following surrounding. Bacteria: unknown Site: Sugar mill effluent outfall (in the sea) The topic of my study is abundance of culturable bacteria at the sugar mill out fall. Thanx!! ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From farrlarr from isu.edu Wed Apr 1 12:42:19 2009 From: farrlarr from isu.edu (Larry Farrell) Date: Wed Apr 1 16:32:25 2009 Subject: [Microbiology] Re: which medium In-Reply-To: References: Message-ID: <4INAl.118664$Rg3.44152@newsfe17.iad> s02001213@student.usp.ac.fj wrote: > > Hi.Please help!! > > Which medium do i use for the cultivation of this particular bacteria in the > following surrounding. > > Bacteria: unknown > Site: Sugar mill effluent outfall (in the sea) > > The topic of my study is abundance of culturable bacteria at the sugar mill out > fall. > > Thanx!! > > ---------------------------------------------------------------- > This message was sent using IMP, the Internet Messaging Program. > Your best bet is to (1) discuss the issue with the instructor who assigned the study (you will be surprised how much information they can provide, and how interested they will in discussing the project with you), and (2) do some digging in the literature (you will be surprised how much you can learn by doing some work on your own, including learning to use the primary literature, an invaluable skill on its own, and even [dare one even hope] possibly learning to do some thinking on your own). Bottom line: Don't expect anyone else to do your homework or your research for you. It isn't in either their or your best interests. -- Larry D. Farrell, Ph.D. Professor Emeritus of Microbiology Idaho State University From arin280 from gmail.com Thu Apr 2 10:15:11 2009 From: arin280 from gmail.com (Arin280) Date: Thu Apr 2 11:29:49 2009 Subject: [Microbiology] A Useful Online Research Website for Biotech Scientists- MyNetResearch.com Message-ID: <574147e5-e7c0-4d79-a6b2-94f474a62b39@r28g2000vbp.googlegroups.com> Dear Researcher, With a team of academics, I recently launched the research website, MyNetResearch.com. Our website helps researchers to increase their research productivity. Specifically, the website enables you to: - manage your research papers and grant proposals online and access them from any location - conduct online surveys and citation searches - receive expert advice on research and statistical design - read about the latest research in hundreds of different subjects - exchange ideas with thousands of other researchers - collaborate with thousands of other researchers on your research papers Currently, over 5,600 researchers from 95 countries and multiple research specialties use MyNetResearch for their research activities. I=92m trying to spread the word about MyNetResearch and to let academic researchers know about this great resource. Regular membership is completely free. I invite you to try MyNetResearch. You can sign up at: https://www.mynetresearch.com/Signup/SignUp.aspx Sincerely, Bay Arinze, Ph.D., Founding Editor MyNetResearch, Empowering Collaboration=99 From limbic_lesion from hotmail.com Thu Apr 2 18:51:16 2009 From: limbic_lesion from hotmail.com (N10) Date: Thu Apr 2 21:17:23 2009 Subject: [Microbiology] Re: re:which medium References: Message-ID: <9o2dnd3wTKfm0kjUnZ2dnUVZ8tmdnZ2d@bt.com> wrote in message news:mailman.706.1238606548.13724.microbio@net.bio.net... > > > Hi.Please help!! > > Which medium do i use for the cultivation of this particular bacteria in > the > following surrounding. > > Bacteria: unknown > Site: Sugar mill effluent outfall (in the sea) > > The topic of my study is abundance of culturable bacteria at the sugar > mill out > fall. > > Thanx!! > > ---------------------------------------------------------------- > This message was sent using IMP, the Internet Messaging Program. > Do you think there might be an abundance of differing organisms ( bacterial Fungal) in such an effluent Do you think there might be both aerobic and anaerobic organisms. I would start with general media to assess the aerobic and anaerobic bacterial populations together with an estimate of the yeast and mould It is by such means you will be able to detrmine if one morphological type predominates. Suggest Plate count agar and Rosebengal CHloramphenicol agar Best N10 From MARCO.BERZANO from ucd.ie Sun Apr 5 05:22:41 2009 From: MARCO.BERZANO from ucd.ie (MARCO BERZANO) Date: Sun Apr 5 12:23:41 2009 Subject: [Microbiology] Workshop announcement - June 3rd-5th 2009 Message-ID: <12EA9661-3502-458C-9CC2-B68BDD69E909@ucd.ie> Dear All, Cryptonet (www.cryptonet.ie) is pleased announce a three day practical workshop on molecular techniques and risk assessment focused on surface water contamination by Cryptosporidium spp. from agricultural sources. All are welcome to join at University College Dublin, Belfield, Dublin, Ireland from Wednesday 3rd to Friday 5th June 2009. The workshop will be hosted by the UCD School of Agriculture, Food Science and Veterinary Medicine, and The Central Veterinary Research Laboratory (DAFF). Participants can choose to join just the one day symposium on molecular methods and risk assessment and, if they wish one of two follow on workshops detailed below. Contamination of surface water by Cryptosporidium spp. A workshop on molecular biological techniques and risk assessment A one day symposium bringing together methodologies in molecular identification of Cryptosporidium spp. and microbial risk assessment will be held on Wednesday June 3rd 2009. A combination of international and local speakers will present on key research issues relating to using molecular tools and risk assessment for Cryptosporidium spp. in agricultural catchments. Attendance at the symposium is welcome without participation in the concurrent practical workshops to be held on June 4th-5th 2009. Workshop 1: Molecular Techniques A two-day practical workshop on molecular biological techniques: towards the development of standard protocols for the detection and quantification of Cryptosporidium oocysts in various environmental matrices. This session will cover the DNA extraction from complex environmental matrices (soil, slurry and contaminated water) and the detection of Cryptosporidium spp. using real time qPCR techniques. Special attention will be dedicated to statistical analysis of real time PCR data. Workshop 2: Microbial Risk Assessment A one-day workshop on risk assessment techniques applied to the study of non point source agricultural pollution. Using a participatory- based approach to teaching and learning, attendees will have the opportunity to use software tools and contribute to in-depth discussions, share experiences, and exchange resources in a variety of thematic topics such as Microbial and Ecological Risk Assessment, Risk Based Site Evaluation, Cryptosporidium Epidemiology. Participants will develop a sound understanding in developing conceptual risk assessment models and semi-quantitative methods in describing microbiological risk. Registration form could be found at www.cryptonet.ie/workshop09/ To request more information contact: marco.berzano@ucd.ie or peter.ziegler@ucd.ie Apologies for cross-posting. _____________________________________________ Dr MARCO BERZANO Bioresources Research Centre / Biosystems Engineering UCD School of Agriculture, Food Science and Veterinary Medicine Agriculture and Food Science Centre University College Dublin Belfield -Dublin 4 -IRELAND Fax: +353 1 7167415 Email: marco.berzano@ucd.ie Skype: esauritorix Mobile: +353 87 0683867 From Harry.Thuis from nvi-vaccin.nl Thu Apr 9 10:08:16 2009 From: Harry.Thuis from nvi-vaccin.nl (Harry Thuis) Date: Thu Apr 9 11:13:59 2009 Subject: [Microbiology] re:which medium Message-ID: We have a problem with the validation of bioburden testing of WFI on R2A medium(milliflex system) We are spiking WFI wih Ps.aeruginosa (in our test the positive controle value was 25 CFU and the value in WFI was < 10 CFU) The recovery is les than 50% . Is this a normal value for this species in WFI? Harry Thuis Nederlands Vaccin Instituut Tel. +31 (0)30-2744334 Fax. +31 (0)30-2744420 E-mail harry.thuis@nvi-vaccin.nl ____________________________________________________________________________ DISCLAIMER: http://www.nvi-vaccin.nl/maildisclaimer.html From LaS from wpunj.edu Thu Apr 9 11:48:40 2009 From: LaS from wpunj.edu (La, Sung) Date: Thu Apr 9 15:55:28 2009 Subject: [Microbiology] teichoplanin Message-ID: Hello: I saw on the Google search that you are looking for way to obtain Teichoplanin. I would like to know that myself and would You kindly let me know if you have found the source where this medicine can be purchased? My email address is : las@wpunj.edu Thank you. From jflood from chem.utoronto.ca Mon Apr 13 13:58:21 2009 From: jflood from chem.utoronto.ca (jflood@chem.utoronto.ca) Date: Mon Apr 13 14:44:12 2009 Subject: [Microbiology] Phe Auxotrophic E. Coli BL21(DE3) Message-ID: <20090413145821.2f9m2ssug4c8w04g@mail.chem.utoronto.ca> Hello, I'm looking for a Phe auxotrophic strain of E Coli that can be used with plasmids with T7 promoter. Preferably BL21 (DE3). Can anybody direct me to a bank, lab or were to buy it? Thnxs From drc.bbsr from gmail.com Mon Apr 20 06:05:27 2009 From: drc.bbsr from gmail.com (drc) Date: Mon Apr 20 09:32:32 2009 Subject: [Microbiology] Summer Training and Guarantee Placement Program @ BioAxis DNA Research Centre Message-ID: <92d6c9c6-4e8f-4392-938c-e002614df5b9@w31g2000prd.googlegroups.com> Summer Training and Guarantee Placement Program @ BioAxis DNA Research Centre BioAxis DNA Research centre is a CRO working in the field of Life science since last 10 years. We are providing trainings in different fields like Bioinformatics, Biotechnology with Mol. Biology, Clinical Research with Data Management. It provides services in different Clinical diagnostics services, Hospital clinical data management, Govt. forensic cases management in Crime identification, Human DNA identification, many Bioinformatics services etc. For further details in services please log on to http://www.bioaxis.in/services.php There is a campus Drive which is going to be held on 25th April to 29th April .In this module we will select students and give them appointment. The selected students will undergo an on job training for 6 months. We are charging 40,000/- for the same. This training is for the Bulk production of desired enzymes, antibodies and whole genome profiling, forensic related services, clinical Research by SAS. We will provide a stipend of Rs4000/- to the selected students in the training period. After the completion of the training, we will send them to the respective unit. .At that time the salary will be Rs 8000-10,000/-. For further assistance please log on to www.dnares.in, mail us at drc.bbsr@gmail.com or call on 09238338983. From alirezabedeltavana from yahoo.com Fri Apr 24 23:34:29 2009 From: alirezabedeltavana from yahoo.com (ALIREZA BEDELTAVANA) Date: Sat Apr 25 12:01:10 2009 Subject: [Microbiology] Fw: lipolytic and porteolytic Message-ID: <335898.21499.qm@web45110.mail.sp1.yahoo.com> ----- Forwarded Message ---- From: ALIREZA BEDELTAVANA To: Microbiology Sent: Monday, 20 April, 2009 17:47:04 Subject: lipolytic and porteolytic ________________________________ Hi everybody I'd like to evaluate the lipolytic and porteolytic characters of Pseudomonas fluorescens strains in different tempereture and I'm looking for some good methods or culture media formulation. Thanks in advance for your help. ________________________________ From trioct_angelia from yahoo.com Mon Apr 27 10:31:55 2009 From: trioct_angelia from yahoo.com (Tri O.A. Samosir) Date: Mon Apr 27 11:46:28 2009 Subject: [Microbiology] Want to join this milis Message-ID: <559968.64317.qm@web30005.mail.mud.yahoo.com> Hello, my name is Tri, a student of Food Science and Technology , Bogor Agricultural University. Now I'm doing my final research at Surveillance institution (BPOM) about isolation and identification pathogens that can cause fodd disease. May I know which forum discussion could I join to share about some research in microbiology?Cause in my country is too hard to get the literature (thanks before) There was some question, that I couldn't find the answer when I have been asked to experiment with the reagents. Maybe you can help me, please... Why serotyping of Salmonella enterica O and H antigen is needed? what is the overplus result doing this stage if we used identification by API 20E system. As we know API 20E described the subspecies too, doesn't it? So by API we knew the serovar without serotyping stage. But why in WHO GSS protocol still use serotyping after identification by API. Is it to proof something? Thank you for the explanation Warmest regards, Tri Octora Angelia Department of Food Science and Technology Bogor Agricultural University Ph. +62 856 2244 554 trioct_angelia@yahoo.com From zzhao from vcu.edu Mon Apr 27 12:27:38 2009 From: zzhao from vcu.edu (Zhongming Zhao) Date: Mon Apr 27 19:15:43 2009 Subject: [Microbiology] Call for Abstracts/Papers, Third Summit on Systems Biology Message-ID: <49F5EB0A.105@vcu.edu> +++++++++++++++++++++++++++++++++++++++++++++ This email and the attached documents are intended to serve as an invitation for you and your colleagues to actively participate in a conference, the third Summit on Systems Biology, which will be held June 16-19, 2009 in Richmond, Virginia. This will be the third in a planned continuing series of Summits on System Biology, with different themes each year. In 2007, our theme was 'Basic, Clinical and Translational Research' with a focus on heart disease and cancer. We received broad support for the 2007 conference and the response from attendees was excellent. This year our focus is Microbial Systems Biology and we invite you to participate. details are provided below. Submit your abstract online and join us!! *Abstract submission deadline: May 7, 2009* *SUMMIT ON SYSTEMS BIOLOGY 2009: The Microbial World and Beyond June 16-19, 2009* The Third Annual Summit on Systems Biology will be held on June 16-19, 2009, in historic Richmond, Virginia located two hours south of Washington, DC. The Summit is comprised of five scientific sessions and two workshops to bring together computational and experimental scientists in the area of microbial systems. The third summit will discuss research directions and latest findings in the "omics" domain, as well as promote collaborations in microbial systems biology and related disciplines. Dr. Leroy Hood, Director of the Institute for Systems Biology, and Dr. Stuart Kauffman, of the University of Calgary, and a recipient of the MacArthur Foundation Award, and Albert-L?szl? Barab?si, Director, Center for Complex Network Research, Northeastern University, all pioneers in Systems Biology, are members of the Summit Steering Committee. *The five scientific sessions are: * . Microbial Engineering . Metagenomics and Microbial Ecology . Host-Pathogen Interaction . Human Microbiome . Technological Advances in Systems Biology *The two workshops are:* . Tree of Life and Microbial Systems Biology . Gene Networks and Diseases *Program Design* The three day program is divided into five (5) main sessions and two (2) workshops. The workshops will be held on the day preceding the formal opening of the conference. Web will also host two poster sessions, one for students and one for more senior researchers. There will be a */New Technology Workshop /*as a pre-conference event, in concert with the student poster session on Tuesday evening, June 16. Instrument manufacturers will provide presentations and virtual demonstrations on the latest technology and applications. *Technical Symposia* *Session I: Microbial Engineering * The first session provides a broad overview of synthetic biology, systems biology and biological engineering and their applications to health, clean and renewable energy, and the environment. Specific topics include synthetic gene networks and the biosynthetic capacity of microbial systems. This session brings together a diverse group of participants from a variety of disciplines. *Session II: Metagenomics and Microbial Ecology* The second session of the summit highlights the latest developments in our understanding of the emergence and evolution of pathogenic and non-pathogenic microbes with a particular emphasis on insights gained through genomic analyses of microbial communities. *Session III: Host-Pathogen Interactions* The third session focuses on how innovative approaches and high-throughput techniques are used to study the complex interactions between bacterial pathogens and their eukaryotic hosts. Participants studying a range of pathogens using various host systems come together to discuss integrated systems approaches. *Session IV: Human Microbiome* The fourth session provides the opportunity to discuss the latest advances in sequencing the human microbiome. The session underlines the importance of the metabolic function of microbiota and the role of symbiosis in health and disease. *Session V: Technological Advances in Systems Biology * The final session focuses on how emerging technologies are driving advances in metagenomics, transcriptomics, proteomics and metabolomics at the microbial community level. The session features cutting-edge research on quantitative mass spectrometry and the latest advances in DNA sequencing. *Interactive Workshops* *New Technology Workshop *This event will involve presentations on the latest technology and applications by instrument manufacturers and active researchers on the opening evening of the conference, and will be held concurrently with the student poster session and reception. *Workshop I: Gene networks and disease *The past decade has witnessed an exponential growth of biological data including genomic sequences, gene annotations, expression and regulation, and protein-protein interactions. We will include discussions on the relationship between oral pathogens and cardiovascular disease, with special interest in the virulence of Streptococcus sanguinis. This workshop focuses on the reconstruction and analysis of gene networks and pathways of human pathogens and their application to disease. *Workshop II: Assembling the Microbial Tree of Life *During the 21^st century, the advances on microbial systems biology will be closely tied to phylogenomics approaches to unravel the evolutionary relationships of the microbial world. By involving an interdisciplinary group of scientists and students from outside the tree of life community, including mathematicians, statisticians, clinicians, computer scientists, chemists, physicists, and engineers, the workshop will foster the development of new questions and lines of research contributing to the generation of a robust microbial phylogenetic framework. *Venue and Accommodations:* The Omni Richmond Hotel 100 South 12th Street Richmond, Virginia 23219 Phone: (804) 344-7000, Fax: (804) 648-6704 Toll Free 1-888-444-OMNI The Omni Richmond Hotel, a luxury hotel, offers Southern hospitality in a contemporary setting. Overlooking the scenic James River in the heart of Richmond's financial and historical districts, the Omni Richmond is located in lively, historic Shockoe Slip. _http://www.omnihotels.com/FindAHotel/Richmond.aspx_ */Government rate for Summit attendees for early registration./* For conference information and registration, go to: _http://www.vcu.edu/csbc/systemsbiologysummit/_ Program contact: Zhongming Zhao, Ph.D., Assistant Professor Psychiatric Genetics Email: _zzhao@vcu.edu_ Gregory A. Buck, Ph.D.Chair, Steering Committee Director, Center for the Study of Biological Complexity, Virginia Commonwealth University. By phone: 804-827-0026 or email _sysbiosummit@vcu.edu_ -- ============================================ Zhongming Zhao, Ph.D. Asst. Professor of Bioinformatics Depts. Psychiatry and Human Genetics and Center for the Study of Biological Complexity Virginia Commonwealth University PO Box 980126, Richmond VA 23298-0126 Phone: (804) 828-8129 Fax: (804) 828-1471 http://bioinfo.vipbg.vcu.edu/ From dugrad from nospam.com Tue Apr 28 11:10:07 2009 From: dugrad from nospam.com (JEDilworth) Date: Tue Apr 28 12:38:32 2009 Subject: [Microbiology] Re: Candida culturing References: Message-ID: Plate your diluent. You probably have a contaminant. Also, when setting up from ATCC, it's best to make a couple of passes to "wake up" your organism before working with it. Sub a colony from your original plate to a new SABS (or similar medium) and let grow. Do this again. Put your bug onto a slant to save it (or freeze it using beads, which I don't know how to do but someone else may). You can also do it the "old way" we used to do - put on a slant and overlay with sterile mineral oil. Keeps for about a year at room temperature that way. Hope this helps. Judy Dilworth, M.T. (ASCP) Microbiology "Christopher M Havens" wrote in message news:mailman.981.1217627990.3533.microbio@net.bio.net... Hello, I have been having some trouble maintaining cultures of candida which I have aquired from ATCC. From Matthew.Davis from tga.gov.au Tue Apr 28 18:14:01 2009 From: Matthew.Davis from tga.gov.au (Matthew.Davis@tga.gov.au) Date: Tue Apr 28 19:08:02 2009 Subject: [Microbiology] Microbiological Update [SEC=UNCLASSIFIED] Message-ID: Dear Sir/Madam, I was wondering if you would be able to provide me with the details regarding the 'Microbiological Update' journal - as I am interested in possibly subscribing. If you could mail me the details, if possible, this would be most helpful. Yours Faithfully, Matthew Davis BSc (Hons) Auditor - Medicines Audit Team C Office of Manufacturing Quality Therapeutic Goods Administration Level 3, 20 Smith Street Parramatta NSW 2150 Phone: 02 9865 9309 International +61 2 9865 9309 Facsimile: 02 9865 9333 International +61 2 9865 9333 Mobile: 0432 756 794 Email: matthew.davis@tga.gov.au ******************************************************************************* "Important: This transmission is intended only for the use of the addressee and may contain confidential or legally privileged information and has been sent in accordance with the TGA security policy. If you are not the intended recipient, you are notified that any use or dissemination of this communication is strictly prohibited. If you receive this transmission in error please notify the author Immediately and delete all copies of this transmission." ******************************************************************************** From limbic_lesion from hotmail.com Thu Apr 30 13:27:51 2009 From: limbic_lesion from hotmail.com (N10) Date: Thu Apr 30 14:40:51 2009 Subject: [Microbiology] Re: Want to join this milis References: Message-ID: "Tri O.A. Samosir" wrote in message news:mailman.1057.1240850824.13724.microbio@net.bio.net... > Hello, > my name is Tri, a student of Food Science and Technology , Bogor > Agricultural University. > Now > I'm doing my final research at Surveillance institution (BPOM) about > isolation and identification pathogens that can cause fodd disease. > > May > I know which forum discussion could I join to share about some research > in microbiology?Cause in my country is too hard to get the literature > (thanks before) > > There > was some question, that I couldn't find the answer when I have been > asked to experiment with the reagents. Maybe you can help me, please... > Why serotyping of Salmonella enterica O and H antigen is needed? what is > the overplus result doing this stage if we used identification by API 20E > system. > As we know API 20E described the subspecies too, doesn't it? So by API > we knew the serovar without serotyping stage. But why in WHO GSS > protocol still use serotyping after identification by API. Is it to > proof something? > Thank you for the explanation > > > > Warmest regards, Hello Tri Most robust protocols such as ISO standard require both Serological and Biochemical identification of Salmonella cultures isolated from foods. As you probabely know there several Thousands of differing Salmonella species , not to mention sub species. Classic biochemical tests inbclude Fermentation reactions ( also H2S production ) and LDC ( decraboxylation reactions) as produced with Lysine Iron agar and Triple sugar Iron agar slopes and butts. In many labs these combined test systems have been replaced by strips which extend the range of tests. The problem is that by only biochemical using test it is imposibile to actually speciate a Salmonella isolate. An Api strip will not speciate say Salmonella agona or Salmonella seftenberg. Such strips apply broad well established probabilites to reaction profiles which is an indication of Genus rather than a specific confirmation or any other taxinomic status ( such as species). Many Enterobacteriaceae cross react on Biohemical pannels either beacuse of their intrinsic genetic make up of by posseion of plassmids. So for example AP 20 E strips can give low differentiation between numerous Citrobacter species and Salmonella thus illustration the weakness of relying purely on bichemical identification. IT is imoprtant to be as certain as possible that one has isolated Salmonella from a food product as obviously there is probable impact on human health. Additonally in some countries Sero Group A Salmonella may be isolated. This group includes Typhoid and it is imperative the correct identification to species level is achieved. For epidemiological studies one also needs eaxct typing of Salmonella isolates which cannot ever be attained simply by biochemical profiling. SOme biochemical reactions such as variation in the ability to ferment lactose can be usefull in epidemiological work. Serological identification allows typing to many sthousands of Salmonella species and serovars . By conducting a few agglutionation reacts it is possible to fisrtly confirm the isolate as Salmonella ( PLoy O Poly H reactions) and then wdifferentiate any particular isolate to SERO GROUP level ( commonly A-S although there are more). Most labs can support the purchase of Poly H/O antisera and Group specific antisera. To actually speciate an isolate requires species specific agglutination factors;of which there are dozens. Most food labs would not stock ALL species specific anti sera but would rely on a regional center of expertice for eaxt specication of an isolate. The message is API 20 E is other forms biochemically panneling cannot be soley relied on to obtain a confident postive identification of a Salmonella culture. Hope this helps Mail me privately if you requirte further assistance Bets N10 > > Tri Octora Angelia > Department of Food Science and Technology > Bogor Agricultural University > Ph. +62 856 2244 554 > trioct_angelia@yahoo.com > > > From dugrad from nospam.com Thu Apr 30 14:23:23 2009 From: dugrad from nospam.com (JEDilworth) Date: Thu Apr 30 18:11:03 2009 Subject: [Microbiology] Re: Want to join this milis References: Message-ID: N10 is exactly right. I have had automated systems call an organism Salmonella or Shigella, and API call it something else. We are required in our laboratory to confirm with serological agglutinations before we report these bugs. Too much hangs on these results, since these are enteric pathogens reported to the state health department and ultimately wind up in CDC's statistics. We then send the isolate to the state health department for further serogrouping. It is expensive to maintain lots of different antisera so we are going to carry only the Poly and Vi Antisera in the future and leave it up to the state to further group the isolate. Judy Dilworth, M.T. (ASCP) Microbiology "N10" wrote in message news:rdidnatszNA0cGTUnZ2dnUVZ8oCdnZ2d@bt.com... > > The message is API 20 E is other forms biochemically panneling cannot be > soley relied on to obtain a confident postive identification of a > Salmonella culture. > > Hope this helps > > Mail me privately if you requirte further assistance > > Bets N10 From bactitech from hortonsbay.com Thu Apr 30 14:24:52 2009 From: bactitech from hortonsbay.com (JEDilworth) Date: Thu Apr 30 18:11:08 2009 Subject: [Microbiology] Re: Want to join this milis References: Message-ID: N10 is exactly right. I have had automated systems call an organism Salmonella or Shigella, and API call it something else. We are required in our laboratory to confirm with serological agglutinations before we report these bugs. Too much hangs on these results, since these are enteric pathogens reported to the state health department and ultimately wind up in CDC's statistics. We then send the isolate to the state health department for further serogrouping. It is expensive to maintain lots of different antisera so we are going to carry only the Poly and Vi Antisera in the future and leave it up to the state to further group the isolate. Judy Dilworth, M.T. (ASCP) Microbiology "N10" wrote in message news:rdidnatszNA0cGTUnZ2dnUVZ8oCdnZ2d@bt.com... > > The message is API 20 E is other forms biochemically panneling cannot be > soley relied on to obtain a confident postive identification of a > Salmonella culture. > > Hope this helps > > Mail me privately if you requirte further assistance > > Bets N10 From limbic_lesion from hotmail.com Thu Apr 30 17:30:29 2009 From: limbic_lesion from hotmail.com (N10) Date: Thu Apr 30 18:11:21 2009 Subject: [Microbiology] Re: Want to join this milis References: Message-ID: OMG ! someone actually bothers to use , let alone heard of Vi anti sera....There really are some actual, microbiologists left and operating on this planet :):):) May the light in culturescontinue to flouresce :) All the best N10 "JEDilworth" wrote in message news:pOKdnXDcn6avZmTUnZ2dnUVZ_rwAAAAA@buckeye-express.com... > N10 is exactly right. I have had automated systems call an organism > Salmonella or Shigella, and API call it something else. We are required in > our laboratory to confirm with serological agglutinations before we report > these bugs. Too much hangs on these results, since these are enteric > pathogens reported to the state health department and ultimately wind up > in > CDC's statistics. > > We then send the isolate to the state health department for further > serogrouping. It is expensive to maintain lots of different antisera so we > are going to carry only the Poly and Vi Antisera in the future and leave > it > up to the state to further group the isolate. > > Judy Dilworth, M.T. (ASCP) > Microbiology > > "N10" wrote in message > news:rdidnatszNA0cGTUnZ2dnUVZ8oCdnZ2d@bt.com... >> >> The message is API 20 E is other forms biochemically panneling cannot be >> soley relied on to obtain a confident postive identification of a >> Salmonella culture. >> >> Hope this helps >> >> Mail me privately if you requirte further assistance >> >> Bets N10 > > > > > From limbic_lesion from hotmail.com Thu Apr 30 17:53:00 2009 From: limbic_lesion from hotmail.com (N10) Date: Thu Apr 30 20:09:07 2009 Subject: [Microbiology] Re: lipolytic and porteolytic References: Message-ID: Alireza CAn you tell us what it is that you wish to investigate specifcally ? For example are you interested in specific substrates or generally just want to show catabolic activity or comptetance of the strains. How many strains do you have and how do you know they are actually different strain s? Best N10 "ALIREZA BEDELTAVANA" wrote in message news:mailman.1040.1240679108.13724.microbio@net.bio.net... ----- Forwarded Message ---- From: ALIREZA BEDELTAVANA To: Microbiology Sent: Monday, 20 April, 2009 17:47:04 Subject: lipolytic and porteolytic ________________________________ Hi everybody I'd like to evaluate the lipolytic and porteolytic characters of Pseudomonas fluorescens strains in different tempereture and I'm looking for some good methods or culture media formulation. Thanks in advance for your help. ________________________________ From yjgent from nospamcox.net Thu Apr 30 20:05:04 2009 From: yjgent from nospamcox.net (John Gentile) Date: Thu Apr 30 20:47:06 2009 Subject: [Microbiology] Re: Want to join this milis References: Message-ID: <2009043021050416807-yjgent@nospamcoxnet> The true microbiologists are a rare breed indeed! I remember having a full compliment of sera for both Salmonella and Shigella, but as Judy says, it got too expensive and we relegated typing to the state. I did want to add to the discussion about the API strips and automated instruments replacing traditional agars and biochemical tests. A long time ago (in the 70's in another lab) we had a demo by the API rep and she included a rare E. coli that the API successfully identified. This thing was H2S positive and in many ways resembled Salmonella. We were quite impressed and the API strips soon replaced my hand made agar slants. Then here in my present lab (staffed by rotating techs and automated equipment) I was following one of those techs who was apparently working up a Salmonella. But on closer look by me I realized that this might be that rare H2S positive E. coli. I sent it to the state lab and they went crazy over it trying every sera in their kits. Finally the supervisor called me and asked me if I was trying to trick them. I knew it was an E. coli, just wanted them to experience it too. Technology cannot truly replace an experienced microbiologist, and the techs today look more to technology to give them answers. So to answer Tri, you can't take shortcuts and rely too much on technology. -- John Gentile MS, M(ASCP) Ancillary Applications Informatics Spc. VA Medical Center Providence, RI On 2009-04-30 18:30:29 -0400, "N10" said: > OMG ! someone actually bothers to use , let alone heard of Vi anti > sera....There really are some actual, microbiologists left and operating on > this planet :):):) > > May the light in culturescontinue to flouresce :) > > > > All the best > > N10 > > > > "JEDilworth" wrote in message > news:pOKdnXDcn6avZmTUnZ2dnUVZ_rwAAAAA@buckeye-express.com... >> N10 is exactly right. I have had automated systems call an organism >> Salmonella or Shigella, and API call it something else. We are required in >> our laboratory to confirm with serological agglutinations before we report >> these bugs. Too much hangs on these results, since these are enteric >> pathogens reported to the state health department and ultimately wind up >> in >> CDC's statistics. >> >> We then send the isolate to the state health department for further >> serogrouping. It is expensive to maintain lots of different antisera so we >> are going to carry only the Poly and Vi Antisera in the future and leave >> it >> up to the state to further group the isolate. >> >> Judy Dilworth, M.T. (ASCP) >> Microbiology >> >> "N10" wrote in message >> news:rdidnatszNA0cGTUnZ2dnUVZ8oCdnZ2d@bt.com... >>> >>> The message is API 20 E is other forms biochemically panneling cannot be >>> soley relied on to obtain a confident postive identification of a >>> Salmonella culture. >>> >>> Hope this helps >>> >>> Mail me privately if you requirte further assistance >>> >>> Bets N10 From farrlarr from isu.edu Thu Apr 30 21:40:29 2009 From: farrlarr from isu.edu (Larry Farrell) Date: Fri May 1 12:11:08 2009 Subject: [Microbiology] Re: Want to join this milis In-Reply-To: <2009043021050416807-yjgent@nospamcoxnet> References: <2009043021050416807-yjgent@nospamcoxnet> Message-ID: John Gentile wrote: > The true microbiologists are a rare breed indeed! I remember having a > full compliment of sera for both Salmonella and Shigella, but as Judy > says, it got too expensive and we relegated typing to the state. > [snip] > > Technology cannot truly replace an experienced microbiologist, and the > techs today look more to technology to give them answers. So to answer > Tri, you can't take shortcuts and rely too much on technology. Not to be completely Luddite, but there is a fine line between doing what the kit says and knowing what the kit results (and everything else about the kit) mean. Unfortunately, way too many people these days know only how to use the kit, according to directions in most cases, with absolutely no understanding of what the kit actually does or why/how it does it. This doesn't apply just to the clinical or technical lab but to virtually all of microbiology/molecular biology. It is sort of like teaching to the test (which is another pet peeve of mine); it really doesn't make any difference whether or not the student/employee has any knowledge of the background of the kit, as long as they can shove sample in one end and get data out the other. In my personal case, I well remember learning electron microscopy on an Hitachi HU-11A, where the electron gun and lenses had to be aligned by hand and focusing was highly dependent on direct observation of diffraction fringes. Knowing how to do all that was probably an issue in my getting the job at Idaho State, where they had just purchased an Hitachi HU-11B and had no one who knew how to run it. Later, the HU-11B was replaced with a Zeiss EM9, which does pretty much everything automatically, and we rapidly began to turn out students who could do the sample preparation and get pictures that gave good data with essentially no knowledge of how the machine operated (no matter how much you preach about operational functions, if they don't have to actually do the operations, the students dump that information pretty quickly). Ah, we are all going to Hell in a handbasket!! -- Larry D. Farrell, Ph.D. Professor Emeritus of Microbiology Idaho State University