[Microbiology] Re: re:which medium

N10 via microbio%40net.bio.net (by limbic_lesion from hotmail.com)
Thu Apr 2 18:51:16 EST 2009


<s02001213 from student.usp.ac.fj> wrote in message 
news:mailman.706.1238606548.13724.microbio from net.bio.net...
>
>
> Hi.Please help!!
>
> Which medium do i use for the cultivation of this particular bacteria in 
> the
> following surrounding.
>
> Bacteria: unknown
> Site: Sugar mill effluent outfall (in the sea)
>
> The topic of my study is abundance of culturable bacteria at the sugar 
> mill out
> fall.
>
> Thanx!!
>
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Do you think there might be an abundance of differing organisms ( bacterial 
Fungal) in such an effluent

Do you think there might be both aerobic and anaerobic organisms.


I would start with general media to assess  the aerobic and anaerobic 
bacterial populations together with an estimate of the yeast and mould

It is by such means you will be able to detrmine if one morphological type 
predominates.

Suggest Plate count agar  and Rosebengal CHloramphenicol agar


Best

N10






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