[Microbiology] Question on Enumeration of PAH degrading bacteria

Shalini selvam via microbio%40net.bio.net (by lovey5dovey from hotmail.com)
Sun Dec 6 07:23:49 EST 2009


Enumeration of PAH degrading bacteria can be based on turbidity in spectrophotometry readings. But how to avoid any turbidity related to PAH itself?
My readings are not consistent and when there is high turbidty,basically i think its because of the oil that has been pipetted together during sampling from flask. Moreover, the use of blank here doesnt really solve the problem for me to assume there is no interference from oil.

Secondly, can a free floating powder medium eg bushnell haas, that are not 100% dissolved can interrupt the turbidity also? 

Hope to get a hand!

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