[Microbiology] Suggestion for primer designing for expression

Akihiro Ueda via microbio%40net.bio.net (by akiu0325 from hotmail.com)
Wed Jul 22 22:35:03 EST 2009

Restriction site for NcoI has ATG=2C=20
so you can use this ATG in frame to start codon of your gene.
Make sure your gene does not have NcoI site.

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