[Microbiology] Re: E.coli MC4100 thi- genotype

Tom Knight via microbio%40net.bio.net (by tk from shaggy.csail.mit.edu)
Mon Mar 9 15:49:41 EST 2009


> "Julian Spagnuolo" <julianspagnuolo from hotmail.com> wrote in message 
> I'm trying to create a pspA reporter plasmid by linking the psp
> promoter using the thi- genotype of E.coli MC4100 as an auxotrophic
> marker, however I'm having 'a little' trouble finding out which gene
> has actually been deleted or mutated so that I can incorporate it into
> my plasmid.
> Does anyone out there know what gene the thi- in E.coli MC4100 actually is?

You might check the E. coli genetic stock center listings:
http://cgsc.biology.yale.edu/Strain.php?ID=9973

They don't list this strain as thi-, but it could be part of the three
deletion regions.


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