From bactitech from hortonsbay.com Sat May 2 00:20:45 2009 From: bactitech from hortonsbay.com (JEDilworth) Date: Sat May 2 11:37:13 2009 Subject: [Microbiology] Re: Want to join this milis References: <2009043021050416807-yjgent@nospamcoxnet> Message-ID: <-a6dnVf12IOyRWbUnZ2dnUVZ_tGdnZ2d@buckeye-express.com> Sorry about the duplicate posts. We had to get a new computer and I just set up newsgroups with the ISP. I didn't think the messages had gone out so I kept trying. I think the days of the "real" microbiologists will be gone in another generation. Our laboratory has rapidly, and quite rightly so, embraced new molecular technology for a number of tests. We are adding on more and will soon be testing for Campylobacter in stools via PCR and not on culture plates any more. We had added the new PNA FISH testing to our "rapid" testing to determine if septic patients with gram positive cocci in clusters in positive blood cultures have Staph. aureus or not within a couple of hours after discovering the positive. Differentiating this from coagulase negative Staph. (which is most usually a contaminant) can save hospitals and patients about three days in the hospital. The list goes on. I learned my reactions on tubed media. Most of our newer techs have seen a few tubed reactions, and memorize ImVic's for the registry, but the reality is that Vitek2 is doing the bulk of our identifications for gram negatives (and other bugs) in a few hours in 64 well cards that are read automatically. Microbes are characterized now not by their biochemicals but their genetics. Yes, Larry, we are becoming has-beens. N10 - thanks so much for the compliment. They were talking about getting rid of Vi and just using Poly+Vi to screen, but I "mentioned" that without Vi by itself you wouldn't know if the Poly+Vi agglutination was due to Poly or Vi. The Vi is staying. Judy Dilworth, M.T. (ASCP) Microbiology "Larry Farrell" wrote in message news:xitKl.4155$0S.1002@newsfe22.iad... > > Ah, we are all going to Hell in a handbasket!! From kdevik from gmail.com Sun May 3 04:22:36 2009 From: kdevik from gmail.com (Kanchanadevi k) Date: Sun May 3 14:12:04 2009 Subject: [Microbiology] hi Message-ID: <8b3d015d0905030222l40ef1d2bgbc5571de5864ffdf@mail.gmail.com> hi Is there anyone who do field trial on the infected plants by using bacterial culture i have some doubt in doing experiment in field so can anyone please help me in this regard thanks in advance with regards K.Kanchana Devi, Research Scholar, From limbic_lesion from hotmail.com Tue May 19 18:34:05 2009 From: limbic_lesion from hotmail.com (N10) Date: Tue May 19 22:45:18 2009 Subject: [Microbiology] Re: Want to join this milis References: <2009043021050416807-yjgent@nospamcoxnet> <-a6dnVf12IOyRWbUnZ2dnUVZ_tGdnZ2d@buckeye-express.com> Message-ID: "JEDilworth" wrote in message news:-a6dnVf12IOyRWbUnZ2dnUVZ_tGdnZ2d@buckeye-express.com... > Sorry about the duplicate posts. We had to get a new computer and I just > set up newsgroups with the ISP. I didn't think the messages had gone out > so I kept trying. > > I think the days of the "real" microbiologists will be gone in another > generation. Our laboratory has rapidly, and quite rightly so, embraced new > molecular technology for a number of tests. We are adding on more and will > soon be testing for Campylobacter in stools via PCR and not on culture > plates any more. We had added the new PNA FISH testing to our "rapid" > testing to determine if septic patients with gram positive cocci in > clusters in positive blood cultures have Staph. aureus or not within a > couple of hours after discovering the positive. Differentiating this from > coagulase negative Staph. (which is most usually a contaminant) can save > hospitals and patients about three days in the hospital. The list goes on. > > I learned my reactions on tubed media. Most of our newer techs have seen a > few tubed reactions, and memorize ImVic's for the registry, but the > reality is that Vitek2 is doing the bulk of our identifications for gram > negatives (and other bugs) in a few hours in 64 well cards that are read > automatically. Microbes are characterized now not by their biochemicals > but their genetics. > > Yes, Larry, we are becoming has-beens. > > N10 - thanks so much for the compliment. They were talking about getting > rid of Vi and just using Poly+Vi to screen, but I "mentioned" that without > Vi by itself you wouldn't know if the Poly+Vi agglutination was due to > Poly or Vi. The Vi is staying. > > Judy Dilworth, M.T. (ASCP) > Microbiology > > > "Larry Farrell" wrote in message > news:xitKl.4155$0S.1002@newsfe22.iad... >> >> Ah, we are all going to Hell in a handbasket!! > > Judy :) you are more than welcome to loads of compliments and thanks for the dialogue on Vi...if I have any more kids Im gonna call one Vi ...that wont happen because of the vasectomy lol so maybe my next cat will called Vi :) Anyway this is an interesting thread to me an as employer of microbiologists. I would have to report the IQ of candidate microbiologists seems observably up over ( as it does for most eductated young people) the last decade but actual knowledge of microbiology and the ability to think ones way out of a microbiological waste bag seems down in my estimate ; which seems to agree with the kit culture model. Im really fortunate I have three real Microbilogists in my Diagnostic section ( Food Microbiology). They all have 13 years experience with my Company and I would have to say that they unequivocabley know their job 100 % inside out. They are so good they leave auditors and me speechless...Im blessed. These days we simply cannot employ people like them unless its a blue moon or an eight day week ! Im all for creating jobs in Science for almost anyone but the trend to "off the shelf" technologies for critical measurments really focuses the need for good scientists capable of producing robust validations of such systems prior to adoption. Fortunately in Europe and certainly in the UK the Food microbiological industry is higly regulated by the requirment for laboratories to obtain accredition to BS EN 17025. For any methodolgy to be approved under this regime requires a robust validation and demonstartion of personnel competance against measurable standards and external audits of proficiency. Under such rigors useless kits, methods,technologies and sometimes people tend to disappear. So it seems atleast in some areas of microbiology there is a compensating mechanism for shallowness. An area of interest to me is that of Diagnostic confidence in the end user of diagnistic information. In the medical field ( not mine) I perceive extreme proficiency and confidence in the result obtained with modern analytical techniques ( as you report). In my own country, in realtion to food microbiology, we ineveitabley get into massive debates of whether or not the correct result has been obtained with a given new technique be it PCR,Vitek identification or the use of monoclonal Latex agglutination systems when a event occurs threatening Public health. For some reason ( even none microbiologists ) seem to gravitate towards confirmation of pathogens, obtained by any route, via pure culture isolation and conventional or classical microbiological identification technologies. I would have to admit Im on that boat too. N10 M.Sc,B.Sc.Mi.biol.Ci.biol...grade III piano :) From bactitech from hortonsbay.com Mon May 25 23:22:29 2009 From: bactitech from hortonsbay.com (JEDilworth) Date: Tue May 26 00:32:53 2009 Subject: [Microbiology] Re: Want to join this milis References: <2009043021050416807-yjgent@nospamcoxnet> <-a6dnVf12IOyRWbUnZ2dnUVZ_tGdnZ2d@buckeye-express.com> Message-ID: <4rydnUs43dgf84bXnZ2dnUVZ_uOdnZ2d@buckeye-express.com> Thanks, N10. Sorry I haven't been monitoring this NG for a bit. I do want to make a correction to my previous post: we will be running Campylobacter in stools by an EIA procedure, NOT PCR. However, we WILL be doing C. difficile toxins by PCR. I'm starting to get trained on this procedure tomorrow. I like the idea of calling a cat Vi! Judy Dilworth, M.T. (ASCP) Microbiology "N10" wrote in message news:a_OdnVv7wKBz3I7XnZ2dnUVZ8tqdnZ2d@bt.com... > > Judy :) you are more than welcome to loads of compliments and thanks for > the dialogue on Vi...if I have any more kids Im gonna call one Vi > ...that wont happen because of the vasectomy lol so maybe my next cat > will called Vi :) > > Anyway this is an interesting thread to me an as employer of > microbiologists. From bumblebeeboy from mindspring.com Wed May 27 02:15:13 2009 From: bumblebeeboy from mindspring.com (Bumble B. Boy) Date: Wed May 27 11:30:46 2009 Subject: [Microbiology] Bacterium candidodestruens Message-ID: Hi all, I have run across a rather obscure reference to a bacterium isolated in 1956 that attacks Candida albicans, and I wish to obtain it. Akiba, T and Iwata, K. On the destructive invasion of a new species, "Bacterium candidodestruens" into Candida cells. 1954. Japanese Journal of Experimental Medicine. 24(3) pp.159-166 I have sent a letter to the University of Tokyo, Dept. of Bacteriology(sic), but I suspect the authors may be retired or deceased. I have checked ATCC but it was never deposited there. Does anyone have this bacterium (pigmented gram negative rods) or know how I can go about locating it? Please e-mail replies since I do not regularly read this newsgroup. Thanks! Allen ### Dear Allen, Did you ever get an answer? I saw the reference also in F.C. Odd's book "Candida and Candidosis: A Review a Bibliography" 2nd Edition. Let me know what you came up with. He thought perhaps from their description that it was probably a Serratia species. Later, Clarke