From jorge1907 from aol.com Thu Oct 1 18:31:14 2009 From: jorge1907 from aol.com (jorge1907@aol.com) Date: Thu Oct 1 20:16:50 2009 Subject: [Microbiology] Re: Microbio Digest, Vol 53, Issue 1 In-Reply-To: <200910011704.n91H4sM08134@net.bio.net> References: <200910011704.n91H4sM08134@net.bio.net> Message-ID: <8CC11066690A07D-4D04-26DED@webmail-d074.sysops.aol.com> agree with limbic - we've enough BS melodrama around already. -----Original Message----- From: microbio-request@oat.bio.indiana.edu To: microbio@magpie.bio.indiana.edu Sent: Thu, Oct 1, 2009 1:04 pm Subject: Microbio Digest, Vol 53, Issue 1 Send Microbio mailing list submissions to microbio@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/microbio or, via email, send a message with subject or body 'help' to microbio-request@net.bio.net You can reach the person managing the list at microbio-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Microbio digest..." Today's Topics: 1. Re: Rising Plague (N10) ---------------------------------------------------------------------- Message: 1 Date: Thu, 1 Oct 2009 00:26:08 +0100 From: "N10" Subject: [Microbiology] Re: Rising Plague To: microbio@net.bio.net Message-ID: You would do better to go get a Doctorate in Pharmacology or Microbiology and do something about this problem you percevive than actually prophiting from writing about it. N10 "sbm" wrote in message news:W9QH9LTC40076.9594328704@reece.net.au... > "Rising Plague: The Global Threat from Deadly Bacteria and > Our Dwindling Arsenal to Fight Them" by Brad Spellberg > (Amazon.com: http://xrl.us/RisingPlague ) > > (M&C) - Meant as a serious wakeup call to professionals and > laypersons alike, this is a well researched yet > approachable examination of a frightening future as the > adaptability of bacteria threatens to overtake humanity's > ability to create effective antibiotics. As drug companies > continue to focus on the more lucrative markets of erectile > dysfunction or overactive bladders, the number of new > antibiotics under development has dwindled to a pitiful > few. This, at a time when numerous strains of bacteria have > developed alarming resistance to every antibiotic currently > available and kill over 100,000 Americans annually.. > > Continued: http://xrl.us/RisingPlague2 > > > ------------------------------ _______________________________________________ Microbio mailing list Microbio@net.bi o.net http://www.bio.net/biomail/listinfo/microbio End of Microbio Digest, Vol 53, Issue 1 *************************************** From genenazareth from hotmail.com Fri Oct 2 09:58:14 2009 From: genenazareth from hotmail.com (genevieve nazareth) Date: Fri Oct 2 14:36:54 2009 Subject: [Microbiology] (no subject) Message-ID: I am using an E. coli BL21(DE3) clone with IPTG as an inducer. However though there is significant expression at the flask level, very little or no expression in 1L fed batch fermentations. Any suggestions? _________________________________________________________________ Live Search extreme As India feels the heat of poll season, get all the info you need on the MSN News Aggregator http://news.in.msn.com/National/indiaelections2009/aggregator/default.aspx From cherie_parker from amsinomedusa.com Mon Oct 5 10:41:10 2009 From: cherie_parker from amsinomedusa.com (Cherie Parker) Date: Mon Oct 5 12:38:15 2009 Subject: [Microbiology] Rodac Plates Message-ID: We use Rodac (contact) plates for our environmental monitoring. Is there a method to estimate TNTC plates? How do you read spreader colonies on a contact plate? Is there a standard method for counting colonies on a Rodac plate? Cherie Parker Amsino Medical USA 1150 Antioch Pike, Suite 200 Nashville, TN 37211 Ph:615-332-9959 Fax:615-831-1817 cherie_parker@amsinomedusa.com From yjgent from nospamcox.net Tue Oct 6 18:49:53 2009 From: yjgent from nospamcox.net (John Gentile) Date: Tue Oct 6 20:22:48 2009 Subject: [Microbiology] Re: Rodac Plates References: Message-ID: <2009100619495316807-yjgent@nospamcoxnet> On 2009-10-05 11:41:10 -0400, "Cherie Parker" said: > We use Rodac (contact) plates for our environmental monitoring. Is there a > method to estimate TNTC plates? How do you read spreader colonies on a > contact plate? Is there a standard method for counting colonies on a Rodac > plate? > > > > Cherie Parker > > Amsino Medical USA > > 1150 Antioch Pike, Suite 200 > > Nashville, TN 37211 > > Ph:615-332-9959 > > Fax:615-831-1817 > > cherie_parker@amsinomedusa.com > > > > I thought contact plates went out with bleeding times. I don't think there are any clear meaninful guidelines for how to quantify plates. What may be an acceptable count now may be TNTC in a few hours. The environment is rather dirty so what is the purpose? To try to educate someone on how to clean? It might be more important to examine what cleaning solutions they are using. Most cleaning crews I've run into seem to make the strongest formula they can instead of going by the manufacturers directions. How many times have you walked into a public bathroom and been assaulted by the fumes of the disinfectant? If a solution works best at 2% it doesn't work better at 10 or 20%, but look at what they are doing. Even in our lab, most of our bleach solutions were much more than the 5% recommended. After washing a bench down, it sometimes smelled like an over chlorinated pool! We stopped doing any environmental cultures because no one could interpret them. -- John Gentile MS, M(ASCP) Laboratory Information Mgr. VA Medical Center Providence, RI yjgent@cox.net From jorge1907 from aol.com Wed Oct 7 17:04:01 2009 From: jorge1907 from aol.com (jorge1907@aol.com) Date: Wed Oct 7 19:45:07 2009 Subject: [Microbiology] Re: Microbio Digest, Vol 53, Issue 5 In-Reply-To: <200910071708.n97H8fM25311@net.bio.net> References: <200910071708.n97H8fM25311@net.bio.net> Message-ID: <8CC15B1366A1166-17F0-18F7@webmail-d053.sysops.aol.com> Unlike my arrogant colleague?- I know contact plates are in current and common?use in hygienic manufacturing application and counts are related to the surface area of contact with the contact plates.? The data are quirte relevant to clean room hygiene. No - as is obvious - there is no way to take TNTC? to a quantitation.??If you experience spreaders - you take the same precautions as in any culture - medium that diminshes, early counts, even overlay (tho you need a steady hand). -----Original Message----- From: microbio-request@oat.bio.indiana.edu To: microbio@magpie.bio.indiana.edu Sent: Wed, Oct 7, 2009 1:08 pm Subject: Microbio Digest, Vol 53, Issue 5 Send Microbio mailing list submissions to microbio@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/microbio or, via email, send a message with subject or body 'help' to microbio-request@net.bio.net You can reach the person managing the list at microbio-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Microbio digest..." Today's Topics: 1. Re: Rodac Plates (John Gentile) ---------------------------------------------------------------------- Message: 1 Date: Tue, 6 Oct 2009 19:49:53 -0400 From: John Gentile Subject: [Microbiology] Re: Rodac Plates To: microbio@net.bio.net Message-ID: <2009100619495316807-yjgent@nospamcoxnet> Content-Type: text/plain; charset=ISO-8859-1; format=flowed On 2009-10-05 11:41:10 -0400, "Cherie Parker" said: > We use Rodac (contact) plates for our environmental monitoring. Is there a > method to estimate TNTC plates? How do you read spreader colonies on a > contact plate? Is there a standard method for counting colonies on a Rodac > plate? > > > > Cherie Parker > > Amsino Medical USA > > 1150 Antioch Pike, Suite 200 > > Nashville, TN 37211 > > Ph:615-332-9959 > > Fax:615-831-1817 > > cherie_parker@amsinomedusa.com > > > > I thought contact plates went out with bleeding times. I don't think there are any clear meaninful guidelines for how to quantify plates. What may be an acceptable count now may be TNTC in a few hours. The environment is rather dirty so what is the purpose? To try to educate someone on how to clean? It might be more important to examine what cleaning solutions they are using. Most cleaning crews I've run into seem to make the strongest formula they can instead of going by the manufacturers directions. How many times have you walked into a public bathroom and been assaulted by the fumes of the disinfectant? If a solution works best at 2% it doesn't work better at 10 or 20%, but look at what they are doing. Even in our lab, most of our bleach solutions were much more than the 5% recommended. After washing a bench down, it sometimes smelled like an over chlorinated pool! We stopped doing any environmental cultures because no one could interpret them. -- John Gentile MS, M(ASCP) Laboratory Information Mgr. VA Medical Center Providence, RI yjgent@cox.net ------------------------------ _______________________________________________ Microbio mailing list Microbio@net.bio.net http://www.bio.net/biomail/listinfo/microbio End of Microbio Digest, Vol 53, Issue 5 *************************************** From shashemi from pharmascience.com Wed Oct 21 09:40:11 2009 From: shashemi from pharmascience.com (Saeid Hashemi Tabatabaei) Date: Wed Oct 21 15:32:11 2009 Subject: [Microbiology] USP Microbial Limits Prep Test Message-ID: Good Day, I read your article and I have a question please? When you perform your validation test, for how long you keep your product in contact with microorganism in your diluents and why? I appreciate your reply in advance. Pharmascience Inc Saeid Hashemi Microbiology Analyst shashemi@pharmascience.com AVIS DE CONFIDENTIALITE : Ce message est confidentiel et est a l'usage exclusif du destinataire. Toute autre personne est par la presente avisee qu'il lui est strictement interdit de le diffuser, distribuer ou reproduire. Si vous avez recu ce message par erreur, veuillez nous en aviser immediatement par retour de courrier electronique et detruire ce message. NOTICE OF CONFIDENTIALITY: This communication is intended only for the party to whom it is addressed and contains confidential information. Any other delivery, distribution, copying or disclosure is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return electronic mail and destroy this message. From richard.price from academia.edu Wed Oct 28 18:33:58 2009 From: richard.price from academia.edu (Richard Price) Date: Wed Oct 28 22:04:03 2009 Subject: [Microbiology] New Academia.edu feature for Microbiology Usenet Message-ID: <9d885662-7abb-4a7b-9b5e-8e6a570648eb@u36g2000prn.googlegroups.com> Dear Microbiology Usenet members, I want to tell you about a new feature on Academia.edu that can connect you with your mailing list. Academia.edu launched 12 months ago and now helps 300,000 academics a month answer the question 'who's researching what?' And now we've built a dedicated page on Academia.edu for the Microbiology Usenet mailing list: http://lists.academia.edu/UsenetBionetMicrobiology Here you'll learn about fellow members of Microbiology Usenet on Academia.edu. You'll be able to see their research interests, papers, and loads of other useful information. Visit the link above, sign up with Academia.edu, and share your research interests with fellow members of the Microbiology Usenet mailing list. Richard Dr. Richard Price, post-doc, Philosophy Dept, Oxford University. Founder of Academia.edu From medanrc from yahoo.com Fri Oct 30 00:19:44 2009 From: medanrc from yahoo.com (Mohamed Eida) Date: Fri Oct 30 11:28:44 2009 Subject: [Microbiology] inquiry about Aspergillus fumigatus and penicillium crustosum Message-ID: <132609.55511.qm@web65504.mail.ac4.yahoo.com> Dear All hope you are fine and doing well I want to ask if there is any record about using Aspergillus fumigatus and penicillium crustosum in composting. if there is any reference please let me know thanks yours Mohamed