Sequencing: Both strands or one?

Bruce Roe broe at aardvark.ucs.uoknor.edu
Tue Jun 16 19:18:00 EST 1992


In article <1992Jun16.132204.21060 at sifon.cc.mcgill.ca>, francis at monod.biol.mcgill.ca writes...
>rrumpf at magnus.acs.ohio-state.edu (Robert Rumpf) writes...
> 
>>>I have a question, more of a survey, really, for anyone doing phylogeny 
>>>studies.  Are you sequencing both strands of whatever gene you are sequencing, 
>>>or just one and assume that's good enough?
>>> 
>>>Bob
>>
> 
> broe at aardvark.ucs.uoknor.edu (Bruce Roe) writes:
> 
>>To insure accuracy of each base, the "rule of three" should be followed.
>>Sequence each base twice in one orientation and once in the other, as a
>>minimum.  I can think of no instance, except for sloppy science, that this
>>rule should not be followed.
>>
> 
> 
>Bruce,
> 
>Is that what they do with the human cDNAs ...   ???   :-j
> 
> 
>francis

I can only speak for what we are doing in my lab.

-bruce
broe at aardvark.ucs.uoknor.edu



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