Archae PCR primers

John Gosink gosink at u.washington.edu
Thu Jul 15 16:30:10 EST 1993


Hi,
				Does anyone have suggestions for PCR primers which would be effective
for amplifying the small subunit ribosomal (16S) gene of archea.  I have
one that corresponds to the 1510-1492 region in E. coli (apparently a
'universal' site), but I need another for the 5' end of the gene.  Also,
would there be any problems if I constructed it with a Not I polylinker on
the end for easier cloning?  Thanks in advance.

																																					-John



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