DNA composition drift expt.

JAB5 at VAXB.YORK.AC.UK JAB5 at VAXB.YORK.AC.UK
Thu Mar 18 14:59:00 EST 1993


Dear Colleagues,
As an afterthought to the %GC thread, has anybody heard
of expts. along the line of...
1. Take mutant host (low/high %GC content)
2. Transform with plasmid+gene of opposite GC trend (high/low)
3. Grow under conds. where expression of the cloned gene is
   essential.
4. Sub-culture ad nauseum.
5. Sequence portion of cloned gene at intervals.

Does a drift to the host GC preference occur?
Over what time-scale?
Probably a common "thought-experiment" which would be
considered too boring to carry out!!
Looking forward to your replies,
Best wishes,
Jim Brannigan
Chemistry Dept.,
University of York
York UK YO1 5DD



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