DNA Amplification

hiscox at uk.ac.afrc hiscox at uk.ac.afrc
Fri Oct 8 03:29:00 EST 1993


Marc,

Most people using a cloning vector, for example, blue script, pUC18/19 etc
to amplify large pieces of DNA. If you want to amplify DNA around 30-40Kb then
use cosmids. If your DNA to be amplified is very large then use a yeast 
artificial chromosome, this can accomodate very large fragments of DNA/
chromosomal material.

Best regards

Julian

E-mail: HISCOX at IRAD.AFRC.AC.UK



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