poor resolution of microsatellites

Pete Rogan rogan at ncifcrf.gov
Sun Feb 13 12:15:03 EST 1994


In article <weixCKMEto.68v at netcom.com> weix at netcom.com (Patrick Weix) writes:
>This question and answer should probably be in Methods&Reagents but
>here goes:
>	I had the same difficulty with resolving allelotypes of simple
>sequence length polymorphisms (SSLPs), some of which differ by just a
>few bases. The solution that was suggested to me was to run the
>samples on a Formamide/Urea Sequencing gel. 
>	For a 100ml tot vol use:
>		42g 	Urea
>		10ml	38%/2% Acyl/Bis
>		10ml	10xTBE
>		40ml	deionized formamide
>		H2O to 100mls.
>
>Don't bother to prerun. Use 500microL of 10% APS and 75 microL of
>TEMED. 

 Just wanted to state my support for Formamide Urea gels as well.

 Pete Rogan
 Div. Genetics
 Dept Pediatrics 
 Penn State Col of Med
 Hershey PA




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