Does anyone have experience with LONG PCR?
kamin_johnson at cellbio.duke.edu
Fri Sep 9 18:24:33 EST 1994
In article <34nl57$fj3 at u.cc.utah.edu>, kelly at thor.med.utah.edu (Kelly
> We are attempting to amplify a 16 kb fragment and would like to hear
> about other's experience with using technique, buffer recipes,
> protocols, and kits tried.
> Any or no success using this, we'd like to know.
> kelly at thor.med.utah.edu
In a recent issue of TIBS (I think, one of the Trends... issues anyway)
there was a discussion of the "long and accurate" PCR technique. The
point was that the technique seems to require one to follow the protocol
of Barnes rigidly. The protocol was given in the issue.
More information about the Mol-evol