Is there experimental proof for Nei & Li (1979)?

Christoph Grunau cgrunau at rkna50.riken.go.jp
Fri Jul 7 03:53:03 EST 1995


Hi RFLPers!

Does anyone knows a paper about the experimental proof for Nei & Li's paper
from 1979 "Mathematical model for studying genetic variations in terms of
restriction endonucleases"?

What I am doing is:

#1) PCR amplification of hsp70 from a mixed population

#2) RFLP of the obtained clones to reduce data redundancy by estimating the
    substitutions per nucleotide site (Nei & Li) and hierarchical
clustering

#3) choosing a type clone for each cluster and sequencing the type clones

#4) sequence comparison

Problem: RFLP and sequence comparison gives SAME tree topology but more
than one order of magnitude DIFFERENT distance values (substitutions per
nucleotide site). The RFLP tree is "smaller" than the sequence tree.

I would appreciate to hear your opinion.

Thanks in advance.

Christoph Grunau



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