primers for rDNA

John J Weiland jweiland at badlands.NoDak.edu
Sat Jun 10 09:45:35 EST 1995


Hello!

I'm interested in finding a pair of primers that would recognize 
conserved regions in the rRNA genes in the mitochondrial, chloroplast and 
nuclear genomes of higher and lower plants.  The idea would be to use 
these primers to PCR amplify a product that would have a different size 
depending on whether that product came from the nuclear, chloroplast or 
mitochondrial DNA.  This could be used as an indirect measure of the 
amount of contamination of prepared nuclear DNA by that from the 
organelles.  Any references would be appreciated.

Thanks!

John



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