Isolating DNA from feathers

Guy Reeves R.Guy.Reeves at ncl.ac.uk
Thu May 18 07:38:44 EST 1995


In article <3paf8k$cvf at due.unit.no>, Jan Sunde <jansunde at stud.unit.no> says:
>
>Could anyone tell me anything at all about isolating DNA from feathers ?
>Methods, practical problems, etc ?
>
Have you seen this article

TI- DNA EXTRACTION, AMPLIFICATION, AND DIRECT SEQUENCING 
FROM HORNBILL 
    FEATHERS
AU- MORIN, PA;MESSIER, J;WOODRUFF, DS
NA- UNIV CALIF DAVIS,DEPT ANTHROPOL,DAVIS,CA,95616
    UNIV CALIF SAN DIEGO,DEPT BIOL,LA JOLLA,CA,92093
    UNIV CALIF SAN DIEGO,CTR MOLEC GENET,LA JOLLA,CA,92093
JN- JOURNAL OF THE SCIENCE SOCIETY OF THAILAND
PY- 1994
VO- 20
NO- 1
PG- 31-41
AB- Two methods for extracting DNA from feathers for non-invasive 
    multilocus genotyping of birds are described DNA was amplified by the 
    polymerase chain reaction and the double-stranded products were 
    sequenced directly without the need for cloning. One method uses a 
    single extraction step with Chelex as the extraction medium thus 
    reducing opportunities for contamination, and eliminating the need to 
    use organic solvents and proteinases. It constitutes a simple, fast and 
    relatively inexpensive method of DNA-level genotyping. Partial 
    mitochondrial cytochrome b sequences of 7 species of hornbill (Aves: 
    Bucerotidae) and a warbler are reported to demonstrate the method's 
    potential for phylogenetic and genealogical analyses involving 
    free-ranging birds and museum specimens.



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