Autoclaves destroy DNA???
skinner at mis.nb.ca
Tue Aug 6 17:11:35 EST 1996
> I have heard diametrically opposed statements about the
> standard steam autoclaves in terms of destroying DNA (on microtubes,
> pestles, etc.) such that PCR will not amplify any DNA that may have been
> the autoclaved items. One group says that 15-20 min in the autoclave at
YES, autoclaving will destroy DNA. To be sure, autoclave small batches for
30-45 minutes. 121C is beyond the melting point of any DNA.
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