Autoclaves destroy DNA???

Barn skinner at mis.nb.ca
Tue Aug 6 17:11:35 EST 1996



> 
>      I have heard diametrically opposed statements about the
effectiveness of
> standard steam autoclaves in terms of destroying DNA (on microtubes,
forceps,
> pestles, etc.) such that PCR will not amplify any DNA that may have been
on
> the autoclaved items.  One group says that 15-20 min in the autoclave at
121C
YES, autoclaving will destroy DNA.  To be sure, autoclave small batches for
30-45 minutes.  121C is beyond the melting point of any DNA.




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