Autoclaves destroy DNA???
chbarry at llnl.gov
Wed Aug 7 12:00:52 EST 1996
Francois Jeanmougin wrote:
> In article <01bb83e4$58093080$47faa4c6 at skinner.mi.net>,
> "Barn" <skinner at mis.nb.ca> writes:
> >YES, autoclaving will destroy DNA. To be sure, autoclave small batches for
> >30-45 minutes. 121C is beyond the melting point of any DNA.
> Well, don't confuse between denaturation and destruction. the
> destruction should come from covalent bond hydrolysis. Then the buffer
> in which you are is important, at least its boiling point and the presence
> of hydrolytic agents.
> Hope this helps,
There are exceptions... If you are really paranoid, there are bacteria
that can handle such temperatures. I don't have a source though.
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