Autoclaves destroy DNA???

Chris Barry chbarry at llnl.gov
Wed Aug 7 12:00:52 EST 1996


Francois Jeanmougin wrote:
> 
> In article <01bb83e4$58093080$47faa4c6 at skinner.mi.net>,
>         "Barn" <skinner at mis.nb.ca> writes:
> 
> >121C
> >YES, autoclaving will destroy DNA.  To be sure, autoclave small batches for
> >30-45 minutes.  121C is beyond the melting point of any DNA.
> >
> 
>         Well, don't confuse between denaturation and destruction. the
> destruction should come from covalent bond hydrolysis. Then the buffer
> in which you are is important, at least its boiling point and the presence
> of hydrolytic agents.
> 
>                                         Hope this helps,
>                      

There are exceptions... If you are really paranoid, there are bacteria
that can handle such temperatures. I don't have a source though.

Chris



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