Autoclaves destroy DNA???

Francois Jeanmougin pingouin at .u-strasbg.fr
Wed Aug 7 05:08:25 EST 1996


In article <01bb83e4$58093080$47faa4c6 at skinner.mi.net>,
	"Barn" <skinner at mis.nb.ca> writes:

>121C
>YES, autoclaving will destroy DNA.  To be sure, autoclave small batches for
>30-45 minutes.  121C is beyond the melting point of any DNA.
>

	Well, don't confuse between denaturation and destruction. the
destruction should come from covalent bond hydrolysis. Then the buffer
in which you are is important, at least its boiling point and the presence
of hydrolytic agents.

					Hope this helps,
						Francois.

-- 
Francois Jeanmougin
Service de bioinformatique / bioinformatics service
IGBMC 
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e-mail : jeanmougin at igbmc.u-strasbg.fr

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