Restriction Enzyme Phylogenies

Daro daro at aol.com
Tue Feb 13 02:50:46 EST 1996


It seems most phylogenies inferred from RE data assay the entire
chromosome.  For instance, I have seen several studies where restriction
fragments are P32 labeled and then analyzed for various "phenotypes". 
However, my research involves a more limited assay...I use an
oligonucleotide probe for a specific gene instead of labeling all the
fragments.  Can I still calculate genetic distance (for example, Nei and
Li's method) from this data or would this distance reflect the specific
gene's (i.e. probed gene) distances?

Ashley,daro at aol.com




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