Modeling Structure By Homology
ram at mbisgi.umd.edu
Fri Jul 5 18:09:39 EST 1996
Douglas Easton (dpeaston at Microagewny.com) wrote:
>I am currently modeling the structue of a protein using an X-ray
>structure for a protein, distantly homologous in sequence and in
>function. I am predicting the secondary structure using ssp, nnsp, GOR
>etc. And alligning sequence homologies and the real and predicted
>secondary structures. The results seem neat but my confidence in the
>approach is a bit shakey.
If it's really distant (how distant is it?), then you're on shaky
ground but I am not sure why you're doing this. Is your goal only to
predict the secondary structure? If so, I doubt any secondary
structure prediction method would be better in general than simply
taking the secondary structure assignment of the homolog according to
a good alignment.
For distant homologs, this is a good thing to do in order to confirm
your alignment. I'd use PHD for predicting strucures also.
>Can you carry this analysis to the tertiary structure level?
Yes, that's what homology modelling is about. I recommend looking at
the November 1995 issue of Proteins: Structure, Function, and Genetics
for some ideas on how to go about doing this, and the pitfalls
me at ram.org || http://www.ram.org || http://www.twisted-helices.com/th
"Religion is the opiate of the masses." ---Karl Marx
"Sex is the opiate of the masses." ---Ram Samudrala
"Television is the opiate of the masses." ---Gerry Goldstein
"Say no to drugs." ---Nancy Reagan
More information about the Mol-evol