amplifiable DNA from museum fish specimens; methods?

Bruce J. Turner fishgen at vt.edu
Wed Jul 17 15:15:04 EST 1996


 Does anyone out there have, or know of, a reliable published protocol for
obtaining amplifiable DNA from fish specimens in museum collections---these
are routinely fixed in "formalin" and subsequently stored in ethanol or
isopropanol.  I need such a protocol to help confirm the genetic identity
of a fish population previously thought to be extinct, but which has
reappeared at its only known locality (there is a serious possibility here
of introductions by humans from nearby, non-endangered populations).  I
haven't found anything, but surely some one has tackled this by now?  Any
help would be appreciated, and sorry for any duplicate mailings...

                        Bruce J. Turner
                        Assoc. Professor of Biology
                        VPISU
                        Blacksburg, VA 24061
                        (540)-231-7444 (V)
                        (540)-231-9307 (F)
                        fishgen at vt.edu






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