DNA ligation help
xcl at med.unc.edu
Sat Jun 1 10:27:52 EST 1996
Hi, there, I have trouble in DNA ligation, the case below:
Insert Fragment: 16.3kb SalI Frag.
Vector: Bscrip-KS(-) SalI cut plasmid or other
salI cut expression vector
It looks like no ligation problem, but in fact, I cannot
success to ligate them and transfer them into DH5a no matter I use
chemical or electroparation method, Have anybody ever meet the same problem?
How can I solve them? Any suggestion is appreciated! Thanks for you attention!
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