finding microsatellites

harley at chempath.uct.ac.za harley at chempath.uct.ac.za
Tue Jun 25 11:57:28 EST 1996


In article <dewolf-2406961651320001 at cauliflower.bio.indiana.edu> 
dewolf at bio.indiana.edu (Diana Wolf) wries:>Path: 

>Are there any companies that will find plant microsatellites (SSRs) and
>design primers for them?  I am working on Datisca glomerata, and need
>microsats, but would prefer to pay someone else to  design them for me. If
>you know how I can contact a company or lab that is willing to do this
>please let me know.

 There is a general need for UNIVERSAL microsats, or at least microsats with a 
broad species range. It is likely that such exist since it merely requires 
that there should be highly conserved sequences nearbye on each side of the 
repeat (and that the repeat dosnt drop out - this may be more of a problem). 
They are probably not that common, but with so many new ones being described a 
few should fall into this category.
So far I do not know of such either for animals or plants, but it 
would not surprise me if a few had already been found but have just not been 
publicised. And publicised they should be since they would help avoid that 
6-month time period to make libraries and screen for candidates (plus the 
expense).
So to generalise the discussion - is there anyone out there who has 
come across such broad-range primer sets ? Also - why not test those of your 
favorites on a wide species range and see if they work ?

Eric Harley, University of Cape Town.



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